Institute of Organic Contaminant Control and Soil Remediation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, PR China.
Soils Department, Faculty of Agriculture, Mansoura University, 35516, Mansoura, Egypt.
Chemosphere. 2023 Oct;337:139287. doi: 10.1016/j.chemosphere.2023.139287. Epub 2023 Jun 23.
Glomalin-related soil protein (GRSP) has gained widespread attention because of its benefits to carbon sequestration, improving soil quality and fixing heavy metals. However, studies on how GRSP affects the environmental fate of organic contaminants are scarce. In this study, different types of GRSPs were isolated from forest soils and characterized to study the binding of GRSPs and PAHs under different environmental conditions. The results indicated that GRSPs contain abundant functional groups (such as -NH, -COOH, and CO) and material composition, like humic acid, proteins, and lipids. For the tested GRSPs, EE-GRSP has lower DOC, SUVA and SUVA values, as well as higher E2/E3 values, indicating that EE-GRSP has lower hydrophobicity and molecular weight. These properties can lead to strong interactions between GRSP and PAHs, especially with benzopyrene, which has a high K and K and a large molecular size, with binding constants ranging from 16,119 to 163,697 L·kg. Furthermore, low pH (pH = 3) and temperature (15 °C) could increase GRSP's aggregation, enhance the GRSP binding ability with PAHs, whose binding constants were 11,595 and 5067.3 L·kg. Therefore, the binding between GRSP and PAHs may lead to changes in the fate of PAHs in the soil and affect the environmental risk of PAHs. The results presented here will deepen our understanding of the environmental function of GRSPs and provide a theoretical basis to further elucidate the mechanisms of GRSPs and organic pollutants.
菌根相关土壤蛋白（GRSP）因其对碳固存、改善土壤质量和固定重金属的益处而受到广泛关注。然而，关于 GRSP 如何影响有机污染物的环境归宿的研究还很少。在这项研究中，从森林土壤中分离出不同类型的 GRSP 并进行了表征，以研究不同环境条件下 GRSP 与 PAHs 的结合情况。结果表明，GRSP 含有丰富的官能团（如-NH、-COOH 和 CO）和物质组成，如腐殖酸、蛋白质和脂质。对于测试的 GRSP，EE-GRSP 具有较低的 DOC、SUVA 和 SUVA 值以及较高的 E2/E3 值，表明 EE-GRSP 具有较低的疏水性和分子量。这些特性可以导致 GRSP 与 PAHs 之间发生强烈的相互作用，特别是与苯并芘，其具有较高的 K 和 K 和较大的分子量，结合常数范围从 16,119 到 163,697 L·kg。此外，低 pH（pH = 3）和温度（15°C）可以增加 GRSP 的聚集，增强 GRSP 与 PAHs 的结合能力，其结合常数分别为 11,595 和 5067.3 L·kg。因此，GRSP 与 PAHs 之间的结合可能导致 PAHs 在土壤中的归宿发生变化，并影响 PAHs 的环境风险。本研究结果将加深我们对 GRSPs 环境功能的理解，并为进一步阐明 GRSPs 与有机污染物的机制提供理论依据。
