Department of Histology and Cytophysiology, Medical University of Bialystok, Mickiewicza 2C Street, 15-222 Bialystok, Poland.
Int J Mol Sci. 2023 Jun 20;24(12):10362. doi: 10.3390/ijms241210362.
The prognosis for patients with RCC is very poor because this cancer is diagnosed mainly in the metastatic stage and is resistant to radio- and chemotherapy. According to recent research, CacyBP/SIP exhibits phosphatase activity against MAPK and may be involved in many cellular processes. This function has not been studied in RCC so far, so we decided to test whether CacyBP/SIP has phosphatase function against ERK1/2 and p38 in high-grade clear cell RCC. The research material consisted of fragments of clear cell RCC, whereas the comparative material consisted of the adjacent normal tissues. Immunohistochemistry and qRT-PCR were used to identify the expression of CacyBP/SIP, ERK1/2, and p38. The studies showed an increase in immunoreactivity and gene expression of the parameters examined in clear cell RCC compared with normal tissues. Only in the case of ERK1/2 was it shown that the expression of the gene was downregulated and the gene was higher in clear cell RCC. These studies demonstrated that CacyBP/SIP lacked phosphatase function against ERK1/2 and p38 in high-grade clear cell RCC. Further research is needed because a better understanding of the role of CacyBP/SIP and MAPK offers hope for the treatment of urological cancer.
RCC 患者的预后非常差，因为这种癌症主要在转移阶段被诊断出来，并且对放射和化学疗法具有抗性。根据最近的研究，CacyBP/SIP 对 MAPK 具有磷酸酶活性，并且可能参与许多细胞过程。到目前为止，尚未在 RCC 中研究该功能，因此我们决定测试 CacyBP/SIP 是否对高级透明细胞 RCC 中的 ERK1/2 和 p38 具有磷酸酶功能。研究材料由透明细胞 RCC 片段组成，而对照材料由相邻的正常组织组成。免疫组织化学和 qRT-PCR 用于鉴定 CacyBP/SIP、ERK1/2 和 p38 的表达。研究表明，与正常组织相比，透明细胞 RCC 中检查的参数的免疫反应性和基因表达增加。仅在 ERK1/2 的情况下，表明基因的表达下调，并且基因在透明细胞 RCC 中更高。这些研究表明，CacyBP/SIP 在高级透明细胞 RCC 中缺乏对 ERK1/2 和 p38 的磷酸酶功能。需要进一步研究，因为更好地了解 CacyBP/SIP 和 MAPK 的作用为治疗泌尿系统癌症带来了希望。
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