Landa-Acuña Daniela, Toro Marcia, Santos-Mendoza Ricardo, Zúñiga-Dávila Doris
Laboratorio de Ecología Microbiana y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima 15024, Peru.
Centro de Ecología Aplicada, Instituto de Zoología y Ecología Tropical, Facultad de Ciencias, Universidad Central de Venezuela, Caracas 1041-A, Venezuela.
Microorganisms. 2023 Jun 16;11(6):1596. doi: 10.3390/microorganisms11061596.
AZO16M2, was characterized for its phosphate solubilization capacity to improve the establishment and survival of var. Valery seedlings under ex-acclimation. Three phosphorus sources (Rock Phosphate (RF), Ca(PO) and KHPO) and two types of substrate (sand:vermiculite (1:1) and Premix N°8) were selected. The factorial analysis of variance ( < 0.05) showed that AZO16M2 (OQ256130) solubilizes Ca(PO) in solid medium, with a Solubilization Index (SI) of 3.77 at 28 °C (pH 6.8). In liquid medium, it was observed that produced 29.6 mg/L soluble P (pH 4.4), and synthesized organic acids (oxalic, D-gluconic, 2-ketogluconic and malic), Indole Acetic Acid (IAA) (33.90 ppm) and siderophores (+). Additionally, acid and alkaline phosphatases (2.59 and 2.56 µg pNP/mL/min) were detected. The presence of the pyrroloquinoline-quinone (PQQ) cofactor gene was confirmed. After inoculating AZO16M2 to in sand:vermiculite with RF, the chlorophyll content was 42.38 SPAD (Soil Plant Analysis Development). Aerial fresh weight (AFW), aerial dry weight (ADW) and root dry weight (RDW) were superior to the control by 64.15%, 60.53% and 43.48%, respectively. In Premix N°8 with RF and , 8.91% longer roots were obtained, with 35.58% and 18.76% more AFW and RFW compared with the control as well as 94.45 SPAD. With Ca(PO), values exceeded the control by 14.15% RFW, with 45.45 SPAD. AZO16M2 favored the ex-climatization of through improving seedling establishment and survival.
对AZO16M2进行了表征，以确定其溶解磷的能力，从而改善瓦莱里变种幼苗在驯化后的定植和存活情况。选择了三种磷源（磷矿粉（RF）、Ca(PO)和KHPO）和两种基质类型（沙子：蛭石（1:1）和预混料8号）。方差分析（<0.05）表明，AZO16M2（OQ256130）在固体培养基中能溶解Ca(PO)，在28°C（pH 6.8）时溶解指数（SI）为3.77。在液体培养基中，观察到其产生了29.6 mg/L的可溶性磷（pH 4.4），并合成了有机酸（草酸、D-葡萄糖酸、2-酮葡萄糖酸和苹果酸）、吲哚乙酸（IAA）（33.90 ppm）和铁载体（+）。此外，还检测到酸性和碱性磷酸酶（2.59和2.56 µg pNP/mL/min）。证实了吡咯喹啉醌（PQQ）辅因子基因的存在。将AZO16M2接种到含有RF的沙子：蛭石中的瓦莱里变种后，叶绿素含量为42.38 SPAD（土壤植物分析发展指数）。地上鲜重（AFW）、地上干重（ADW）和根干重（RDW）分别比对照高出64.15%、60.53%和43.48%。在含有RF和瓦莱里变种的预混料8号中，根长比对照长8.91%，AFW和RFW分别比对照多35.58%和18.76%，叶绿素含量为94.45 SPAD。对于Ca(PO)，根干重比对照高出14.15%，叶绿素含量为45.45 SPAD。AZO16M2通过改善幼苗定植和存活情况，有利于瓦莱里变种的驯化适应。
