Hsiao Jong-Kai, Chen Chih-Lung, Hsieh Wen-Yuan, Kuo Ko-Lin
Department of Medical Imaging, Taipei Tzu Chi Hospital, Buddhist Tzu-Chi Medical Foundation, New Taipei City 23142, Taiwan.
School of Medicine, Tzu Chi University, Hualien 97004, Taiwan.
Pharmaceutics. 2023 Jun 12;15(6):1714. doi: 10.3390/pharmaceutics15061714.
(1) Background: Increasing attention has been given to applying nanosized iron oxide nanoparticles (IOPs) to treat iron deficiency anemia (IDA). Chronic kidney disease (CKD) patients who suffer from IDA often need long-term iron supplements. We aim to evaluate the safety and therapeutic effect of MPB-1523, a novel IOPs, in anemic CKD mice and to monitor iron storage by magnetic resonance (MR) imaging. (2) Methods: MPB-1523 was intraperitoneally delivered to the CKD and sham mice, and blood were collected for hematocrit, iron storage, cytokine assays, and MR imaging throughout the study. (3) Results: The hematocrit levels of CKD and sham mice dropped initially but increased gradually to reach a steady value 60 days after IOP injection. The body iron storage indicator, ferritin gradually rose and total iron-binding capacity stabilized 30 days after IOP injection. No significant inflammation or oxidative stress were observed in both groups. By T2-weighted MR imaging, the liver signal intensity gradually increased in both groups but was more pronounced in the CKD group, indicating aggressive utilization of MPB-1523. MR imaging, histology and electron microscopy showed MPB-1523 is liver-specific. (4) Conclusions: MPB-1523 can serve as a long-term iron supplement and is monitored by MR imaging. Our results have strong translatability to the clinic.
（1）背景：纳米级氧化铁纳米颗粒（IOPs）在治疗缺铁性贫血（IDA）方面受到越来越多的关注。患有IDA的慢性肾脏病（CKD）患者通常需要长期补充铁剂。我们旨在评估新型IOPs——MPB - 1523对贫血CKD小鼠的安全性和治疗效果，并通过磁共振（MR）成像监测铁储存情况。（2）方法：在整个研究过程中，将MPB - 1523腹腔注射给CKD小鼠和假手术小鼠，并采集血液进行血细胞比容、铁储存、细胞因子检测以及MR成像。（3）结果：CKD小鼠和假手术小鼠的血细胞比容水平最初下降，但在注射IOPs后60天逐渐升高并达到稳定值。身体铁储存指标铁蛋白在注射IOPs后30天逐渐上升，总铁结合能力稳定。两组均未观察到明显的炎症或氧化应激。通过T2加权MR成像，两组肝脏信号强度均逐渐增加，但在CKD组中更明显，表明MPB - 1523被积极利用。MR成像、组织学和电子显微镜显示MPB - 1523具有肝脏特异性。（4）结论：MPB - 1523可作为长期铁补充剂，并可通过MR成像进行监测。我们的结果具有很强的临床可转化性。
