Jiménez-Torres Humberto D, Hernández-Olmos Saira L, Reynaga-Delgado Eire, Orozco-Guareño Eulogio
Laboratorio de Fisicoquímica del, Departamento de Química, Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierías, Blvd. Marcelino García Barragán #1451, Guadalajara 44430, Jalisco, Mexico.
Departamento de Farmacobiología, Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierías, Blvd. Marcelino García Barragán #1451, Guadalajara 44430, Jalisco, Mexico.
Polymers (Basel). 2023 Jun 6;15(12):2588. doi: 10.3390/polym15122588.
In this study, lignin was chemically modified to promote hydrogel degradation as a source of carbon and nitrogen for a bacterial consortium consisting of , and, . A hydrogel was synthesized using acrylic acid (AA), acrylamide (AM), and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and cross-linked with the modified lignin. The structural changes and mass loss in the hydrogel, as well as its final composition, were evaluated as functions of the growth of the selected strains in a culture broth with the powdered hydrogel. The average loss was 18.4% wt. The hydrogel was characterized using FTIR spectroscopy, scanning electronic microscopy (SEM), elemental analysis (EA), and thermogravimetric analysis (TGA) before and after bacterial treatment. FTIR showed that the carboxylic groups present in both the lignin and the acrylic acid of the hydrogel decreased during bacterial growth. The bacteria showed a preference for the biomaterial components of the hydrogel. SEM demonstrated superficial morphological changes in the hydrogel. The results reveal that the hydrogel was assimilated by the bacterial consortium while preserving the water retention capacity of the material and that the microorganisms carried out a partial biodegradation of the hydrogel. The results of the EA and TGA confirm that the bacterial consortium not only degraded the biopolymer (lignin), but also used the synthetic hydrogel as a carbon source to degrade its polymeric chains and modified original properties. This modification with lignin as a crosslinker (which is a waste product of the paper industry) is therefore proposed to promote hydrogel degradation.
在本研究中，木质素经过化学改性以促进水凝胶降解，从而为一种由[具体菌株 1]、[具体菌株 2]和[具体菌株 3]组成的细菌群落提供碳源和氮源。使用丙烯酸（AA）、丙烯酰胺（AM）和 2 - 丙烯酰胺基 - 2 - 甲基 - 1 - 丙烷磺酸（AMPS）合成了一种水凝胶，并与改性木质素交联。随着所选菌株在含有粉末状水凝胶的培养液中生长，对水凝胶的结构变化、质量损失及其最终组成进行了评估。平均损失为 18.4%（重量）。在细菌处理前后，使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、元素分析（EA）和热重分析（TGA）对水凝胶进行了表征。FTIR 显示，在细菌生长过程中，木质素和水凝胶丙烯酸中存在的羧基减少。细菌对水凝胶的生物材料成分表现出偏好。SEM 展示了水凝胶表面形态的变化。结果表明，水凝胶被细菌群落同化，同时保留了材料的保水能力，并且微生物对水凝胶进行了部分生物降解。EA 和 TGA 的结果证实，细菌群落不仅降解了生物聚合物（木质素），还将合成水凝胶用作碳源来降解其聚合物链并改变了原始特性。因此，建议使用作为造纸工业废料的木质素作为交联剂进行这种改性，以促进水凝胶降解。
