Sharma Phulwanti Kumari, Kumar Lokesh, Goswami Yamini, Pujani Mukta, Dikshit Madhu, Tandon Ruchi
Translational Health Science and Technology Institute, Faridabad, India.
ESIC Medical College and Hospital, Faridabad, India.
Front Pharmacol. 2023 Jun 12;14:1139654. doi: 10.3389/fphar.2023.1139654. eCollection 2023.
Most critically ill COVID-19 patients have bronchitis, pneumonia, and acute respiratory distress syndrome (ARDS) due to excessive inflammatory conditions. Corticosteroids have largely been prescribed for the management of inflammation in these patients. However, long-term use of corticosteroids in patients with comorbidities such as metabolic, cardiovascular, and other inflammatory disorders is ideally not recommended due to safety issues. A potential and safer anti-inflammatory therapy is therefore the need of the hour. (WS), a well-known herbal medicine used during the pandemic in India to prevent SARS-CoV2 infection, also possesses anti-inflammatory properties. In the present study, we, therefore, evaluated the effect of the aqueous extract of the roots of in the cell-based assays and in the experimental animal models of LPS-induced inflammation. In the NCI-H460, A549 cells and human peripheral blood mononuclear cells (PBMCs) pre-treatment with reduced the LPS-induced expression of the pro-inflammatory cytokines. In addition, extract also showed potent anti-inflammatory activity in the lung tissues of BALB/c mice challenged intranasally with LPS. We observed a marked reduction in the neutrophil counts in the broncho-alveolar lavage (BAL) fluid, inflammatory cytokines, and fibrosis in the mice lungs pre-treated with . Results obtained thus suggest the potential utility of extract in reducing airway inflammation and recommend the clinical evaluation of extract in COVID-19 patients with a high propensity for lung inflammation.
大多数重症 COVID-19 患者因炎症反应过度而患有支气管炎、肺炎和急性呼吸窘迫综合征（ARDS）。皮质类固醇在很大程度上已被用于治疗这些患者的炎症。然而，由于安全问题，理想情况下不建议在患有代谢、心血管和其他炎症性疾病等合并症的患者中长期使用皮质类固醇。因此，当下需要一种潜在且更安全的抗炎疗法。（WS）是印度在疫情期间用于预防 SARS-CoV2 感染的一种知名草药，也具有抗炎特性。因此，在本研究中，我们在基于细胞的试验以及脂多糖（LPS）诱导的炎症实验动物模型中评估了 WS 根的水提取物的作用。在 NCI-H460、A549 细胞和人外周血单核细胞（PBMC）中，用 WS 预处理可降低 LPS 诱导的促炎细胞因子的表达。此外，WS 提取物在经鼻内给予 LPS 攻击的 BALB/c 小鼠的肺组织中也显示出强大的抗炎活性。我们观察到，用 WS 预处理的小鼠支气管肺泡灌洗（BAL）液中的中性粒细胞计数、炎症细胞因子和肺纤维化明显减少。因此，所得结果表明 WS 提取物在减轻气道炎症方面具有潜在效用，并建议对肺部炎症倾向较高的 COVID-19 患者进行 WS 提取物的临床评估。
