Anti-aging & Regenerative Medicine Research Institution, School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, China.
Zibo Mashang Central Hospital, Zhangdian, Shandong, Zibo 255000, China.
Aquat Toxicol. 2023 Aug;261:106621. doi: 10.1016/j.aquatox.2023.106621. Epub 2023 Jun 28.
Rare earth element neodymium (Nd) is widely used in industry and agriculture, which may result in the pollution of aquatic environment. In this study, we exposed zebrafish with 10, 50, and 100 μg/L Nd for four weeks. The results showed that Nd could be accumulated in fish gill and Nd accumulation affected the equilibrium of nutrient elements. Nd decreased the antioxidant enzymes' activity and gene expression level, but enhanced the generation of reactive oxygen species (ROS). Moreover, various concentration of Nd treatments inhibited Nrf2 signaling in gill. To examine the critical role of GSK-3β/Nrf2 signaling on ROS generation under Nd stress, we further interfered gsk-3β gene in zebrafish under 100 μg/L Nd exposure. The result showed that gsk-3β gene interference induced Nrf2 signaling as well as the expression and activity of antioxidant enzymes in fish gill. In all, Nd could be accumulated in fish gill, and the signaling of GSK-3β/Nrf2 was involved in regulating ROS generation under Nd treatments.
稀土元素钕（Nd）广泛应用于工农业，可能导致水环境污染。本研究采用 10、50 和 100μg/L 的钕处理斑马鱼四周，结果显示，钕可在鱼鳃蓄积，且蓄积的钕影响营养元素平衡。钕降低了抗氧化酶的活性和基因表达水平，但增强了活性氧（ROS）的产生。此外，不同浓度的钕处理抑制了鳃组织中 Nrf2 信号通路。为了研究 GSK-3β/Nrf2 信号通路在 Nd 胁迫下 ROS 产生中的关键作用，我们进一步在 100μg/L Nd 暴露下用斑马鱼 gsk-3β 基因干扰实验。结果表明，gsk-3β 基因干扰诱导了鱼鳃组织 Nrf2 信号通路以及抗氧化酶的表达和活性。总之，钕可在鱼鳃蓄积，GSK-3β/Nrf2 信号通路参与调节 Nd 处理下的 ROS 产生。
