Zavafer Alonso, Mancilla Cristian, Jolley Gregory, Murakami Keach
Department of Engineering, Brock University, St. Catharines, ON Canada.
Research School of Chemistry, The Australian National University, Canberra, ACT 2600 Australia.
Biophys Rev. 2023 Apr 4;15(3):385-400. doi: 10.1007/s12551-023-01051-y. eCollection 2023 Jun.
Light is one of the most important factors for photosynthetic organisms to grow. Historically, the amount of light in plant sciences has been referred to as light intensity, irradiance, photosynthetic active radiation, photon flux, photon flux density, etc. On occasion, all these terms are used interchangeably, yet they refer to different physical units and each metric offers distinct information. Even for experts in the fields of plant photobiology, the use of these terms is confusing, and there is a loose implementation of each concept. This makes the use of radiometric units even more confusing to non-experts when looking for ways to measure light, since they could easily feel overwhelmed by the specialized literature. The use of scientific concepts must be accurate, as ambiguity in the use of radiometric quantities can lead to inconsistencies in analysis, thus decreasing the comparability between experiments and to the formulation of incorrect experimental designs. In this review, we provide a simple yet comprehensive view of the use of radiometric quantities in an effort to clarify their meaning and applications. To facilitate understanding, we adopt a minimum amount of mathematical expressions and provide a historical summary of the use of radiometry (with emphasis on plant sciences), examples of uses, and a review of the available instrumentation for radiometric measurements.
光是光合生物生长的最重要因素之一。从历史上看,植物科学中光的量一直被称为光强、辐照度、光合有效辐射、光子通量、光子通量密度等。有时,所有这些术语都可互换使用,但它们指的是不同的物理单位,每个度量提供不同的信息。即使对于植物光生物学领域的专家来说,这些术语的使用也令人困惑,而且每个概念的实施都很宽松。这使得非专家在寻找测量光的方法时,使用辐射测量单位更加困惑,因为他们很容易被专业文献搞得不知所措。科学概念的使用必须准确,因为辐射量使用中的模糊性可能导致分析不一致,从而降低实验之间的可比性,并导致错误的实验设计。在本综述中,我们提供了一个简单而全面的辐射量使用观点,以阐明其含义和应用。为便于理解,我们采用最少的数学表达式,并提供辐射测量使用的历史总结(重点是植物科学)、使用示例以及对现有辐射测量仪器的综述。