School of Environmental and Civil Engineering, Jiangnan University, Wuxi, 214122, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China.
J Environ Manage. 2023 Oct 15;344:118459. doi: 10.1016/j.jenvman.2023.118459. Epub 2023 Jul 1.
Anaerobic digestion (AD) of antibiotic manufacturing wastewater to degrade residual antibiotics and produce mixture of combustible gases has been investigated actively in the past decades. However, detrimental effect of residual antibiotic to microbial activities is commonly faced in AD process, leading to the reduction of treatment efficiency and energy recovery. Herein, the present study systematically evaluated the detoxification effect and mechanism of FeO-modified biochar in AD of erythromycin manufacturing wastewater. Results showed that FeO-modified biochar had stimulatory effect on AD at 0.5 g/L erythromycin existence. A maximum methane yield of 327.7 ± 8.0 mL/g COD was achieved at 3.0 g/L FeO-modified biochar, leading to the increase of 55.7% compared to control group. Mechanistic investigation demonstrated that different levels of FeO-modified biochar could improve methane yield via different metabolic pathways involved in specific bacteria and archaea. Low levels of FeO-modified biochar (i.e., 0.5-1.0 g/L) led to the enrichment of Methanothermobacter sp., strengthening the hydrogenotrophic pathway. On the contrary, high levels of FeO-modified biochar (2.0-3.0 g/L) favored the proliferation of acetogens (e.g., Lentimicrobium sp.) and methanogen (Methanosarcina sp.) and their syntrophic relations played vital role on the simulated AD performance at erythromycin stress. Additionally, the addition of FeO-modified biochar significantly decreased the abundance of representative antibiotic resistant genes (ARGs), benefiting the reduction of environmental risk. The results of this study verified that the application of FeO-modified biochar could be an efficient approach to detoxify erythromycin on AD system, which brings high impacts and positive implications for biological antibiotic wastewater treatment.
过去几十年，人们积极研究抗生素生产废水的厌氧消化（AD），以降解残留抗生素并生产可燃气体混合物。然而，残留抗生素对微生物活性的有害影响在 AD 过程中普遍存在，导致处理效率和能源回收降低。在此，本研究系统评估了 FeO 修饰生物炭对抗生素生产废水 AD 中的解毒效果和机制。结果表明，在 0.5 g/L 红霉素存在下，FeO 修饰生物炭对 AD 具有刺激作用。在 3.0 g/L FeO 修饰生物炭下，最大甲烷产量为 327.7 ± 8.0 mL/g COD，与对照组相比增加了 55.7%。机理研究表明，不同水平的 FeO 修饰生物炭可通过涉及特定细菌和古菌的不同代谢途径提高甲烷产量。低水平的 FeO 修饰生物炭（即 0.5-1.0 g/L）导致 Methanothermobacter sp. 的富集，增强了氢营养途径。相反，高水平的 FeO 修饰生物炭（2.0-3.0 g/L）有利于产乙酸菌（如 Lentimicrobium sp.）和产甲烷菌（Methanosarcina sp.）的增殖，它们的共生关系对模拟 AD 在红霉素胁迫下的性能起着至关重要的作用。此外，添加 FeO 修饰生物炭显著降低了代表性抗生素抗性基因（ARGs）的丰度，有利于降低环境风险。本研究结果验证了 FeO 修饰生物炭在 AD 系统中对抗生素解毒的有效性，这对生物抗生素废水处理具有重要影响和积极意义。
