Sodhi Gurleen Kaur, Saxena Sanjai
Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, Punjab 147004 India.
3 Biotech. 2023 Aug;13(8):262. doi: 10.1007/s13205-023-03679-9. Epub 2023 Jul 3.
In this study, we report the discovery of novel species isolated from the extensively cultivated PUSA 44 rice variety in Punjab, India. Out of the 120 isolates examined, 6.6% and 5% isolates exhibited tolerance to high salinity and drought stress. Isolates 6OSFR2e and 7OSFS3a exhibited the highest indole acetic acid and gibberellic acid production, with 268.32 ± 08.10 and 25.72 ± 0.04 µg/mL. Additionally, isolates 7OSFS3a, 6OSFR2e and 6OSFL4c had highest antioxidant potential with IC 345.45 ± 11.66, 391.58 ± 10.66, and 474.529 ± 11.08 µg/mL. The isolates 6OSFR2e and 6OSFL4c also exhibited phosphate solubilisation with a PI of 1.06 ± 0.00 and 1.04 ± 0.02. The highest cellulase and laccase production with EI 1.24 ± 0.00 and 1.16 ± 0.00 was observed by isolates 6OSFR2e and 6OSFL4c. Promising results were observed in the case of ammonia production. The isolates belonged to the same phylum, Ascomycota and were identified as (6OSFR2e) and (7OSFS3a), and (6OSFL4c) using morpho-taxonomic and molecular identification. The present study provides a critical insight into the characteristics of these species, which could be used to develop a bio-consortium for the rejuvenation of PUSA-44 cultivation.
The online version contains supplementary material available at 10.1007/s13205-023-03679-9.
在本研究中，我们报告了从印度旁遮普邦广泛种植的PUSA 44水稻品种中分离出的新物种。在所检测的120个分离株中，6.6%和5%的分离株表现出对高盐度和干旱胁迫的耐受性。分离株6OSFR2e和7OSFS3a产生的吲哚乙酸和赤霉素含量最高，分别为268.32±08.10和25.72±0.04μg/mL。此外，分离株7OSFS3a、6OSFR2e和6OSFL4c具有最高的抗氧化潜力，IC分别为345.45±11.66、391.58±10.66和474.529±11.08μg/mL。分离株6OSFR2e和6OSFL4c也表现出解磷能力，解磷指数分别为1.06±0.00和1.04±0.02。分离株6OSFR2e和6OSFL4c观察到最高的纤维素酶和漆酶产量，酶活指数分别为1.24±0.00和1.16±0.00。在氨生产方面观察到了有前景的结果。这些分离株属于同一门，子囊菌门，并通过形态分类和分子鉴定被鉴定为（6OSFR2e）、（7OSFS3a）和（6OSFL4c）。本研究对这些物种的特性提供了关键见解，可用于开发一个生物联合体以恢复PUSA - 44的种植。
在线版本包含可在10.1007/s13205 - 023 - 03679 - 9获取的补充材料。
