群体感应基因调控降低了（某种生物）对（按蚊属）（双翅目：蚊科）的吸引力。 （注：原文中部分内容缺失具体生物名称，翻译时用括号标注了缺失部分）
Quorum sensing gene regulation in reduces the attraction of (L.) (Diptera: Culicidae).
作者信息
Kim Dongmin, Crippen Tawni L, Jordan Heather R, Tomberlin Jeffery K
机构信息
Department of Entomology, Texas A&M University, College Station, TX, United States.
Southern Plains Agricultural Research Center, Agricultural Research Service, US Department of Agriculture, College Station, TX, United States.
出版信息
Front Microbiol. 2023 Jun 22;14:1208241. doi: 10.3389/fmicb.2023.1208241. eCollection 2023.
INTRODUCTION
Identifying mechanisms regulating mosquito attraction to hosts is key to suppressing pathogen transmission. Historically, the ecology of the host microbial community and its influence on mosquito attraction, specifically, whether bacterial communication through quorum sensing (QS) modulates VOC production that affects mosquito behavior have not been extensively considered.
METHODS
Behavioral choice assays were applied along with volatile collection, followed by GC-MS and RNA transcriptome analyses of bacteria with and without a quorum-sensing inhibitor, furanone C-30.
RESULTS
Utilizing the quorum-sensing inhibitor on a skin-inhabiting bacterium, , we disrupted its interkingdom communication with adult and mitigated their attraction to a blood-meal by 55.1%.
DISCUSSION
One potential mechanism suppressing mosquito attraction could be the reduction (31.6% in our study) of bacterial volatiles and their associated concentrations by shifting metabolic (12 of 29 up regulated genes) and stress (5 of 36 down regulated genes) responses. Manipulating the quorum-sensing pathways could serve as a mechanism to reduce mosquito attraction to a host. Such manipulations could be developed into novel control methods for pathogen-transmitting mosquitoes and other arthropods.
引言
确定调节蚊子对宿主吸引力的机制是抑制病原体传播的关键。从历史上看，宿主微生物群落的生态学及其对蚊子吸引力的影响，特别是细菌通过群体感应（QS）进行的通讯是否会调节影响蚊子行为的挥发性有机化合物（VOC）的产生，尚未得到广泛研究。
方法
应用行为选择试验并结合挥发性物质收集，随后对添加和不添加群体感应抑制剂呋喃酮C-30的细菌进行气相色谱-质谱联用（GC-MS）和RNA转录组分析。
结果
在一种皮肤栖息细菌上使用群体感应抑制剂，我们破坏了它与成年蚊子之间的跨界通讯，并使它们对血餐的吸引力降低了55.1%。
讨论
抑制蚊子吸引力的一种潜在机制可能是通过改变细菌的代谢（29个上调基因中的12个）和应激（36个下调基因中的5个）反应，减少细菌挥发性物质及其相关浓度（在我们的研究中减少了31.6%）。操纵群体感应途径可以作为一种减少蚊子对宿主吸引力的机制。这种操纵可以发展成为针对传播病原体的蚊子和其他节肢动物的新型控制方法。
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