Departamento de Clínicas de la Reproducción Humana, Crecimiento y Desarrollo Infantil, Doctorado en Ciencias de la Nutrición Traslacional, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
Departamento de Biología Molecular y Genómica, Instituto de Nutrigenética y Nutrigenómica Traslacional, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
Metab Syndr Relat Disord. 2023 Oct;21(8):435-442. doi: 10.1089/met.2023.0014. Epub 2023 Jul 11.
The endocannabinoid system (ECS) plays an integral role in maintaining metabolic homeostasis, where an hyperactivation has been related with serum lipid alterations. The biological effects of ECS are limited by the activation of the endocannabinoid-degrading enzyme fatty acid amide hydrolase (FAAH) and by polyunsaturated fatty acid (PUFA) intake as precursors. The Pro129Thr variant has been associated with obesity in some populations. However, the association with metabolic phenotypes in the Mexican population has not been studied. This study aimed to analyze the association of the Pro129Thr variant with serum lipids and diet in Mexican adults with different metabolic phenotypes. This is a cross-sectional study with 306 subjects between 18 and 65 years of age. They were classified with normal weight (NW) or excess weight (EW) according to their body mass index (BMI). The EW group included individuals with overweight or obesity (BMI 25-39.9 kg/m). The individuals were classified into two metabolic phenotypes, metabolically healthy and metabolically unhealthy (MUH), using the homeostatic model assessment of insulin resistance and the National Cholesterol Education Program-adenosine triphosphate III cutoff points for blood pressure, triglycerides, high-density lipoprotein cholesterol, and fasting glucose. Subjects with ≥2 of 5 altered parameters were classified as MUH. The Pro129Thr variant was determined by allelic discrimination with TaqMan probes. The total cholesterol and very low-density lipoprotein cholesterol levels were associated with the Pro129Thr variant in NW-MUH subjects. Moreover, a lower PUFA intake was found in EW-MUH subjects with the variant. Pro129Thr variant has an important role in lipid metabolism, especially in NW-MUH subjects. By contrast, a low dietary intake of endocannabinoid PUFA precursors may partly counteract the development of the altered lipid profile associated with overweight/obesity.
内源性大麻素系统（ECS）在维持代谢稳态中起着重要作用，其过度激活与血清脂质改变有关。ECS 的生物学效应受到内源性大麻素降解酶脂肪酸酰胺水解酶（FAAH）的激活和多不饱和脂肪酸（PUFA）作为前体的摄入限制。Pro129Thr 变体与一些人群中的肥胖有关。然而，该变体与墨西哥人群代谢表型的关联尚未得到研究。本研究旨在分析 Pro129Thr 变体与不同代谢表型的墨西哥成年人血清脂质和饮食的关系。这是一项横断面研究，共有 306 名 18 至 65 岁的受试者。根据体重指数（BMI）将他们分为正常体重（NW）或超重（EW）。EW 组包括超重或肥胖者（BMI 25-39.9 kg/m）。使用稳态模型评估胰岛素抵抗和国家胆固醇教育计划-三磷酸腺苷 III 血压、甘油三酯、高密度脂蛋白胆固醇和空腹血糖的切点，将个体分为两种代谢表型，即代谢健康和代谢不健康（MUH）。≥5 个参数改变的个体被归类为 MUH。Pro129Thr 变体通过 TaqMan 探针进行等位基因鉴别来确定。在 NW-MUH 受试者中，总胆固醇和极低密度脂蛋白胆固醇水平与 Pro129Thr 变体相关。此外，在 EW-MUH 受试者中发现了较低的 PUFA 摄入量。Pro129Thr 变体在脂质代谢中具有重要作用，尤其是在 NW-MUH 受试者中。相比之下，超重/肥胖相关的改变脂质谱与内源性大麻素 PUFA 前体的低饮食摄入可能部分抵消。
