单细胞转录组分析 rd10 视网膜中的 Müller 胶质细胞。

Single-Cell Transcriptomic Profiling of Müller Glia in the rd10 Retina.

机构信息

Lab for Retinal Cell Biology, Department of Ophthalmology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.

Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.

出版信息

Adv Exp Med Biol. 2023;1415:377-381. doi: 10.1007/978-3-031-27681-1_55.

DOI:10.1007/978-3-031-27681-1_55

PMID:37440060

Abstract

Müller glia are the principal macroglia of the retina and support retinal neurons both in health and disease. In retinitis pigmentosa (RP), a highly heterogeneous inherited retinal disorder, the most common form of pathology involves primary rod degeneration, followed by secondary cone death. To investigate Müller glia responses to rod degeneration, we performed droplet-based single-cell RNA sequencing in the rd10 mouse model of RP during primary rod degeneration. We confirmed known MG behavior on gliosis, metabolic, and immune functions. Pde6b Müller glia also exhibited an increased expression of histocompatibility complex members, which might arise from a novel immune function of Müller glia in RP. We also describe a possible decrease in glial lipid biogenesis, which might affect degenerating photoreceptors.

摘要

Müller 胶质细胞是视网膜的主要大胶质细胞，在健康和疾病状态下均为视网膜神经元提供支持。在色素性视网膜炎（RP）这种高度异质性的遗传性视网膜疾病中，最常见的病理形式涉及原发性杆状细胞变性，随后是次级锥状细胞死亡。为了研究 Müller 胶质细胞对杆状细胞变性的反应，我们在 RP 的 rd10 小鼠模型中进行了基于液滴的单细胞 RNA 测序，该模型处于原发性杆状细胞变性阶段。我们在神经胶质增生、代谢和免疫功能方面证实了已知的 MG 行为。Pde6b Müller 胶质细胞还表现出组织相容性复合体成员的表达增加，这可能源于 Müller 胶质细胞在 RP 中的新型免疫功能。我们还描述了可能的神经胶质细胞脂质生物发生减少，这可能会影响变性的光感受器。