Department of Ophthalmology, Gyeongsang National University College of Medicine, and Gyeongsang National University Hospital, Jinju 52727, Republic of Korea.
Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 52727, Republic of Korea.
Cells. 2023 Jun 23;12(13):1705. doi: 10.3390/cells12131705.
Steroid-induced cataracts (SIC) are defined as cataracts associated with the administration of corticosteroids. Long-term glucocorticoid treatment for inflammatory diseases reportedly increases the risk of SIC, and steroids can induce cataracts by disrupting ocular growth factor balance or homeostasis. In this study, we verified the effect of chondroitin sulfate proteoglycan 5 (CSPG5) using dexamethasone (dexa)-treated human lens epithelial (HLE-B3) cells and the lens epithelium from the anterior capsule of SIC patients obtained during cataract surgery. CSPG5 expression increased in the lens epithelium of SIC patients. The downregulation of CSPG5 suppressed the dexa-induced epithelial-mesenchymal transition (EMT)-related protein expression and motility in HLE-B3 cells. The disruption of the transcription factors and downregulated CSPG5, dexa-induced fibronectin expression, and cell migration in HLE-B3 cells, reaffirming that CSPG5 expression regulates EMT in lens epithelial cells. Taken together, these results indicate that the steroid-induced effects on lens epithelial cells are mediated via alterations in CSPG5 expression. Therefore, our study emphasizes the potential of CSPG5 as a therapeutic target for the prevention and treatment of SIC.
类固醇诱导性白内障（SIC）是指与皮质类固醇药物治疗相关的白内障。据报道，长期用于治疗炎症性疾病的糖皮质激素会增加 SIC 的风险，类固醇可通过破坏眼内生长因子平衡或内稳态来诱导白内障。在这项研究中，我们使用地塞米松（dexa）处理的人晶状体上皮（HLE-B3）细胞和白内障手术中从 SIC 患者前囊获得的晶状体上皮来验证硫酸软骨素蛋白聚糖 5（CSPG5）的作用。CSPG5 在 SIC 患者的晶状体上皮中表达增加。下调 CSPG5 抑制了 HLE-B3 细胞中 dex 诱导的上皮-间充质转化（EMT）相关蛋白表达和运动性。转录因子 和 的破坏下调了 CSPG5、dex 诱导的纤连蛋白表达和 HLE-B3 细胞的迁移，这再次证实了 CSPG5 表达调节晶状体上皮细胞的 EMT。综上所述，这些结果表明，类固醇对晶状体上皮细胞的作用是通过改变 CSPG5 的表达来介导的。因此，我们的研究强调了 CSPG5 作为预防和治疗 SIC 的治疗靶点的潜力。
