Oyarzún-Ruiz Pablo, Thomas Richard S, Santodomingo Adriana M, Uribe Juan E, Ardila Marlon M, Echeverry Diana M, Muñoz-Leal Sebastián, Silva-de la Fuente María C, Loyola Marco, Palma Cristina J, Landaeta-Aqueveque Carlos, Henríquez AnaLía
Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán 3812120, Chile.
Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (MNCN-CSIC), 28006 Madrid, Spain.
Animals (Basel). 2023 Jun 24;13(13):2100. doi: 10.3390/ani13132100.
In Chile, studies of parasites from the family Sarcocystidae (Apicomplexa) have mostly been related to domestic animals. We aimed to assess the presence of Sarcocystidae taxa in cricetid rodents from Central and Southern Chile. We studied 207 rodents, encompassing six species, from 13 localities. We isolated DNA from tissue samples, amplified the Sarcocystidae 18S rRNA gene with polymerase chain reaction, and performed phylogenetic analyses using maximum likelihood and Bayesian inferences. In addition, we examined blood smears and performed histological studies in organs from Sarcocystidae DNA-positive animals. Three specimens were DNA-positive and three genotypes were retrieved and named: sp. P61, related to , was detected in two . Toxoplasmatinae gen. sp. P99 was retrieved from those same two specimens, and was related to and other genera, although it branched independently. sp. R34 was detected in one , and was clustered with congeneric species associated with rodents. No protozoa were found during microscopic studies; thus, it was not possible to confirm parasitic interactions rather than accidental encounters. However, the close relatedness of the retrieved genotypes to parasites of rodents supports the hypothesis of host-parasite associations. All three genotypes are suggested as potential new taxa, including a putative new genus.
在智利，对肉孢子虫科（顶复门）寄生虫的研究大多与家畜有关。我们旨在评估智利中部和南部仓鼠科啮齿动物中肉孢子虫科分类群的存在情况。我们研究了来自13个地点的207只啮齿动物，涵盖6个物种。我们从组织样本中提取DNA，用聚合酶链反应扩增肉孢子虫科18S rRNA基因，并使用最大似然法和贝叶斯推断进行系统发育分析。此外，我们检查了血涂片，并对肉孢子虫科DNA阳性动物的器官进行了组织学研究。三个标本DNA呈阳性，获得并命名了三个基因型：在两个[具体物种]中检测到与[相关物种]有关的P61种。从这两个相同的标本中检索到弓形虫亚科基因型P99，它与[相关物种]和其他属有关，尽管它独立分支。在一个[具体物种]中检测到R34种，它与与啮齿动物相关的同属物种聚类。在显微镜研究中未发现原生动物；因此，无法确认是寄生相互作用而非偶然接触。然而，检索到的基因型与啮齿动物寄生虫的密切相关性支持了宿主 - 寄生虫关联的假设。所有这三个基因型都被认为是潜在的新分类群，包括一个假定的新属。
