Shang Xuejiao, Wei Yabo, Guo Xin, Lei Yongdong, Deng Xiaorong, Zhang Jian
School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
Key Laboratory for Processing and Quality Safety Control of Specialty Agricultural Products of Ministry of Agriculture and Rural Affairs (Provincial and Ministerial Cooperation), School of Food Science and Technology Shihezi University, Shihezi 832003, China.
Foods. 2023 Jun 25;12(13):2479. doi: 10.3390/foods12132479.
In this study, the quality (sensory evaluation, microbial enumerate, color, tvb-n (total volatile basic nitrogen), tca-soluble peptide (trichloroacetic acid-soluble peptide), muscle glucose, lactate, total sugar, Bas (Biogenic amines), VOCs (volatile organic compounds) and the microbial dynamic structure in samples stored at 4 °C were evaluated, and the relationship between VOCs and the diversity structure of microorganisms was also discussed. It was determined by sensory evaluation that the shelf life of samples was around 8 days. Protein and sugar were detected in large quantities by microorganisms in the later stage. At the same time, this also caused a large amount of Bas (biogenic amines) (tyramine, cadaverine, and putrescine). According to high-throughput amplicon sequencing, the initial microbiota of samples was mainly composed of , , , , , , , and . Among them, was the most advantageous taxon of samples at the end of the shelf life. The minor fraction of the microbial consortium consisting of , and was detected. The substances 3-methyl-1-butanol, ethyl acetate, and acetone were the main volatile components. The glucose, lactic acid, and total sugar were negatively correlated with , , , and ; the proportion of these microorganisms was relatively high in the early stage. TVB-N and TCA-soluble peptides were positively correlated with , , , , and , and these microorganisms increased greatly in the later stage. The substance 3-methyl-1-butanol was positively correlated with and negatively correlated with . Ethyl acetate was associated with , and . Acetone was positively correlated with .
在本研究中,对4℃储存样品的品质(感官评价、微生物计数、颜色、挥发性盐基氮、三氯乙酸可溶性肽、肌肉葡萄糖、乳酸、总糖、生物胺、挥发性有机化合物)及微生物动态结构进行了评价,并探讨了挥发性有机化合物与微生物多样性结构之间的关系。通过感官评价确定样品的货架期约为8天。后期微生物大量检测到蛋白质和糖。同时,这也导致了大量生物胺(酪胺、尸胺和腐胺)的产生。根据高通量扩增子测序,样品的初始微生物群主要由、、、、、、、和组成。其中,是货架期结束时样品中最具优势的分类群。检测到由、和组成的微生物群落的一小部分。3-甲基-1-丁醇、乙酸乙酯和丙酮是主要的挥发性成分。葡萄糖、乳酸和总糖与、、、和呈负相关;这些微生物在早期比例相对较高。挥发性盐基氮和三氯乙酸可溶性肽与、、、、和呈正相关,这些微生物在后期大量增加。3-甲基-1-丁醇与呈正相关,与呈负相关。乙酸乙酯与、和有关。丙酮与呈正相关。