College of Horticulture, Jilin Agricultural University, Changchun 130118, China.
College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Int J Mol Sci. 2023 Jun 29;24(13):10883. doi: 10.3390/ijms241310883.
As a major component of the plant primary cell wall, structure changes in pectin may affect the formation of the secondary cell wall and lead to serious consequences on plant growth and development. Pectin-modifying enzymes including pectate lyase-like proteins (PLLs) participate in the remodeling of pectin during organogenesis, especially during fruit ripening. In this study, we used as a model system to identify critical genes that are of particular importance for vascular development. Four genes, named , , and were identified for xylem-specific expression. A knock-out T-DNA mutant of displayed an increased amount of pectin, soluble sugar, and acid-soluble lignin (ASL). Interestingly, the mutant exhibited an irregular xylem phenotype, accompanied by disordered xylem ray cells and an absence of interfascicular phloem fibers. The xylem fiber cell walls in the mutant were thicker than those of the wild type. On the contrary, overexpression resulted in expansion of the phloem and a dramatic change in the xylem-to-phloem ratios. Altogether, our data suggest that as a pectate lyase plays an important role during vascular development in .
作为植物初生细胞壁的主要成分，果胶结构的变化可能会影响次生细胞壁的形成，并对植物的生长和发育产生严重后果。果胶修饰酶，包括果胶裂解酶样蛋白（PLLs），参与器官发生过程中果胶的重塑，特别是在果实成熟过程中。在这项研究中，我们以 为模型系统，鉴定了对血管发育特别重要的关键 基因。鉴定出了 4 个命名为 、 、 和 的基因，它们在木质部中特异性表达。 的敲除 T-DNA 突变体表现出果胶、可溶性糖和酸溶性木质素（ASL）含量增加。有趣的是， 突变体表现出不规则的木质部表型，伴随着木质部射线细胞紊乱和束间韧皮纤维缺失。 突变体木质部纤维细胞壁比野生型厚。相反， 的过表达导致韧皮部扩张和木质部与韧皮部比例的显著变化。总的来说，我们的数据表明， 作为一种果胶裂解酶，在 中的血管发育过程中发挥着重要作用。
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