Center of Safety Production and Testing Technology, China Academy of Safety Science and Technology, Beijing 100012, China.
College of Chemistry, Beijing Normal University, Beijing 100875, China.
Molecules. 2023 Jun 29;28(13):5110. doi: 10.3390/molecules28135110.
Alzheimer's disease (AD), an amyloid-related disease, seriously endangers the health of elderly individuals. According to current research, its main pathogenic factor is the amyloid protein, which is a kind of fibrillar aggregate formed by noncovalent self-assembly of proteins. Based on the characteristics of aggregation-induced emission (AIE), a bislactosyl-decorated tetraphenylethylene (TPE) molecule (TPE + malononitrile + lactose), bearing two malononitrile substituents, was designed and synthesized in this work. The amphiphilic could self-assemble into fluorescent organic nanoparticles (FONs) with near-infrared (NIR) fluorescence emission in physiological PBS (phosphate buffered saline), achieving excellent fluorescent enhancement (47-fold) upon its combination with Aβ fibrils. was successfully applied to image Aβ plaques in the brain tissue of AD transgenic mice, and due to the AIE properties of , no additional rinsing process was necessary. It is believed that the probe reported in this work should be useful for the sensitive detection and accurate localization mapping of Aβ aggregates related to Alzheimer's disease.
阿尔茨海默病（AD）是一种与淀粉样蛋白相关的疾病，严重危害老年人的健康。根据目前的研究，其主要致病因素是淀粉样蛋白，它是一种由蛋白质非共价自组装形成的纤维状聚集物。基于聚集诱导发射（AIE）的特点，本工作设计并合成了一种双乳糖基修饰的四苯乙烯（TPE）分子（TPE+丙二腈+乳糖），该分子带有两个丙二腈取代基。两亲性的可以在生理 PBS（磷酸盐缓冲盐水）中自组装成具有近红外（NIR）荧光发射的荧光有机纳米粒子（FONs），与 Aβ 纤维结合后可实现优异的荧光增强（47 倍）。成功地将用于 AD 转基因小鼠脑组织中 Aβ 斑块的成像，由于的 AIE 特性，无需额外的冲洗过程。相信本工作中报道的探针对于与阿尔茨海默病相关的 Aβ 聚集物的灵敏检测和准确定位具有重要意义。
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