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转录组和蛋白质组联合分析为石榴花瓣化研究提供见解。

Combined Transcriptome and Proteome Analysis Provides Insights into Petaloidy in Pomegranate.

作者信息

Huo Yan, Yang Han, Ding Wenjie, Huang Tao, Yuan Zhaohe, Zhu Zunling

机构信息

College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China.

Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China.

出版信息

Plants (Basel). 2023 Jun 21;12(13):2402. doi: 10.3390/plants12132402.

DOI:10.3390/plants12132402
PMID:37446962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10346766/
Abstract

Petaloidy leads to a plump floral pattern and increases the landscape value of ornamental pomegranates; however, research on the mechanism of petaloidy in ornamental pomegranates is limited. In this study, we aimed to screen candidate genes related to petaloidy. We performed transcriptomic and proteomic sequencing of the stamens and petals of single-petal and double-petal flowers of ornamental pomegranates. Briefly, 24,567 genes and 5865 proteins were identified, of which 5721 genes were quantified at both transcriptional and translational levels. In the petal and stamen comparison groups, the association between differentially abundant proteins (DAPs) and differentially expressed genes (DEGs) was higher than that between all genes and all proteins, indicating that petaloidy impacts the correlation between genes and proteins. The enrichment results of transcriptome, proteome, and correlation analyses showed that cell wall metabolism, jasmonic acid signal transduction, redox balance, and transmembrane transport affected petaloidy. Nine hormone-related DEGs/DAPs were selected, among which ARF, ILR1, LAX2, and JAR1 may promote petal doubling. Sixteen transcription factor DEGs/DAPs were selected, among which EREBP, LOB, MEF2, MYB, C3H, and trihelix may promote petal doubling. Our results provide transcriptomic and proteomic data on the formation mechanism of petaloidy and a theoretical basis for breeding new ornamental pomegranate varieties.

摘要

花瓣化导致石榴花型饱满，提高了观赏石榴的景观价值；然而，关于观赏石榴花瓣化机制的研究有限。在本研究中，我们旨在筛选与花瓣化相关的候选基因。我们对观赏石榴单瓣花和重瓣花的雄蕊和花瓣进行了转录组和蛋白质组测序。简要地说，共鉴定出24567个基因和5865种蛋白质，其中5721个基因在转录和翻译水平均被定量。在花瓣和雄蕊比较组中，差异丰富蛋白质（DAP）与差异表达基因（DEG）之间的关联性高于所有基因与所有蛋白质之间的关联性，表明花瓣化影响基因与蛋白质之间的相关性。转录组、蛋白质组和相关性分析的富集结果表明，细胞壁代谢、茉莉酸信号转导、氧化还原平衡和跨膜运输影响花瓣化。筛选出9个与激素相关的DEG/DAP，其中ARF、ILR1、LAX2和JAR1可能促进花瓣加倍。筛选出16个转录因子DEG/DAP，其中EREBP、LOB、MEF2、MYB、C3H和三螺旋可能促进花瓣加倍。我们的结果提供了关于花瓣化形成机制的转录组和蛋白质组数据，为培育新的观赏石榴品种提供了理论依据。

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