Liebers Monique, Hommel Elisabeth, Grübler Björn, Danehl Jakob, Offermann Sascha, Pfannschmidt Thomas
Pflanzenphysiologie, Institut für Botanik, Naturwissenschaftliche Fakultät, Gottfried-Wilhelm-Leibniz-Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany.
Plants (Basel). 2023 Jun 25;12(13):2442. doi: 10.3390/plants12132442.
are small freshwater plants with extraordinary high growth rates. We aimed to test whether this correlates with a more efficient photosynthesis, the primary energy source for growth. To this end, we compared photosynthesis properties of the duckweed and the terrestrial model plant . Chlorophyll fluorescence analyses revealed high similarity in principle photosynthesis characteristics; however, exhibited a more effective light energy transfer into photochemistry and more stable photosynthesis parameters especially under high light intensities. Western immunoblot analyses of representative photosynthesis proteins suggested potential post-translational modifications in proteins that are possibly connected to this. Phospho-threonine phosphorylation patterns of thylakoid membrane proteins displayed a few differences between the two species. However, phosphorylation-dependent processes in such as photosystem II antenna association and the recovery from high-light-induced photoinhibition were not different from responses known from terrestrial plants. We thus hypothesize that molecular differences in photosynthesis proteins are associated with yet unidentified mechanisms that improve photosynthesis and growth efficiencies. We also developed a high-magnification video imaging approach for multiplication which is useful to assess the impact of external factors on photosynthesis and growth.
是具有极高生长速率的小型淡水植物。我们旨在测试这是否与更高效的光合作用相关，光合作用是生长的主要能量来源。为此，我们比较了浮萍和陆生模式植物的光合作用特性。叶绿素荧光分析揭示了光合作用基本特征的高度相似性；然而，浮萍表现出更有效地将光能转化为光化学能，并且光合作用参数更稳定，尤其是在高光强度下。对代表性光合作用蛋白的蛋白质免疫印迹分析表明，浮萍蛋白可能存在潜在的翻译后修饰，这可能与此有关。类囊体膜蛋白的磷酸苏氨酸磷酸化模式在这两个物种之间显示出一些差异。然而，浮萍中依赖磷酸化的过程，如光系统II天线缔合和高光诱导光抑制后的恢复，与陆生植物已知的反应并无不同。因此，我们推测浮萍光合作用蛋白的分子差异与尚未确定的提高光合作用和生长效率的机制有关。我们还开发了一种用于浮萍繁殖的高倍视频成像方法，这有助于评估外部因素对浮萍光合作用和生长的影响。
