Filyushin Mikhail A, Anisimova Olga K, Shchennikova Anna V, Kochieva Elena Z
Research Center of Biotechnology, Institute of Bioengineering, Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow 119071, Russia.
Plants (Basel). 2023 Jul 3;12(13):2538. doi: 10.3390/plants12132538.
Dehydration-responsive element-binding (DREB) transcription factors (TFs) of the A1 and A2 subfamilies involved in plant stress responses have not yet been reported in species. In this study, we used bioinformatics and comparative transcriptomics to identify and characterize A1 and A2 genes redundant in garlic ( L.) and analyze their expression in cultivars differing in the sensitivity to cold and infection. Eight A1 () and eight A2 () genes were identified. genes located in tandem on chromosome 1 had similar expression patterns, suggesting functional redundancy. were scattered on different chromosomes and had organ- and genotype-specific expressions. and promoters contained 7 and 9 hormone- and stress-responsive -regulatory elements, respectively, and 13 sites associated with TF binding and plant development. In both -resistant and -sensitive cultivars, fungal infection upregulated the , , , , and genes and downregulated , but the magnitude of response depended on the infection susceptibility of the cultivar. Cold exposure strongly upregulated the genes, but downregulated most genes. Our results provide the foundation for further functional analysis of the DREB TFs in crops and could contribute to the breeding of stress-tolerant varieties.
参与植物应激反应的A1和A2亚家族脱水响应元件结合（DREB）转录因子在该物种中尚未见报道。在本研究中，我们利用生物信息学和比较转录组学来鉴定和表征大蒜（ ）中冗余的A1和A2基因，并分析它们在对冷害和感染敏感性不同的品种中的表达情况。共鉴定出8个A1（ ）基因和8个A2（ ）基因。位于1号染色体上串联排列的 基因具有相似的表达模式，表明功能冗余。 基因分散在不同染色体上，具有器官和基因型特异性表达。 和 基因的启动子分别包含7个和9个激素和应激响应调控元件，以及13个与转录因子结合和植物发育相关的位点。在抗病和感病品种中，真菌感染均上调了 、 、 、 、 和 基因的表达，下调了 基因的表达，但响应程度取决于品种的感染易感性。冷处理强烈上调了 基因的表达，但下调了大多数 基因的表达。我们的研究结果为进一步对大蒜作物中DREB转录因子进行功能分析奠定了基础，并可能有助于培育耐逆品种。
