病例报告：一例由引起的眼部感染。

Case report: A case of ocular infection caused by .

机构信息

Clinical Laboratory, The General Hospital of Western Theater Command, Chengdu, China.

出版信息

Front Cell Infect Microbiol. 2023 Jun 28;13:1160831. doi: 10.3389/fcimb.2023.1160831. eCollection 2023.

DOI:10.3389/fcimb.2023.1160831

PMID:37448776

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10338080/

Abstract

OBJECTIVE

The aim of this study is to identify the pathogen causing ocular infection in a Chinese patient and to describe its morphological characteristics.

METHODS

Samples from the patient's intraoperative pus were collected for microscopic examination and culture. Morphology and drug sensitivities of the isolated fungus were analyzed. Ribosomal DNA (rDNA) sequencing was performed and blasted in GenBank.

RESULTS

A strain of fungi was repeatedly isolated from pus samples in different types of medium. No conidia were shown when the isolate cultured on normal PDA medium, whereas pseudoseptate thick-walled conidia were shown when cultured on medium containing leaf leachate. The results of BLAST and phylogenetic trees based on internal transcribed spacer, beta-tubulin, translation elongation factor 1-alpha, and RNA polymerase II gene demonstrated that the isolated fungus was . Minimum inhibitory concentration results of this organism were as follows: anidulafungin, 0.06 μg/ml; amphotericin B, 0.12 μg/ml; micafungin, 0.06 μg/ml; caspofungin, 0.5 μg/ml; 5-fluorocytosine, >64 μg/ml; posaconazole, 2 μg/ml; voriconazole, 0.25 μg/ml; itraconazole, 0.5 μg/ml; fluconazole, 64 μg/ml.

CONCLUSION

The case was infected with and led to eye suppurative endophthalmitis and blindness. Combined applications of morphological and molecular biology techniques facilitate accurate diagnosis of fungal infections.

摘要

目的

本研究旨在鉴定一位中国患者眼部感染的病原体，并描述其形态特征。

方法

采集患者术中脓液进行显微镜检查和培养。分析分离真菌的形态和药敏特性。核糖体 DNA（rDNA）测序并在 GenBank 中进行比对。

结果

从不同类型培养基的脓液样本中反复分离出一株真菌。在普通 PDA 培养基上培养时，该分离株未见分生孢子，而在含有叶浸出液的培养基上培养时，可见假隔膜厚壁分生孢子。BLAST 结果和基于内部转录间隔区、β-微管蛋白、翻译延伸因子 1-α 和 RNA 聚合酶 II 基因的系统发育树表明，分离出的真菌为。该菌的最低抑菌浓度结果如下：安尼拉fungin，0.06μg/ml；两性霉素 B，0.12μg/ml；米卡fungin，0.06μg/ml；卡泊芬净，0.5μg/ml；5-氟胞嘧啶，>64μg/ml；泊沙康唑，2μg/ml；伏立康唑，0.25μg/ml；伊曲康唑，0.5μg/ml；氟康唑，64μg/ml。