Department of Plant Pathology, Washington State University, Pullman, WA, USA.
Methods Mol Biol. 2023;2690:1-8. doi: 10.1007/978-1-0716-3327-4_1.
Protein-protein interactions are specific and direct physical contact between two or more proteins, and the interaction involves hydrogen bonding, electrostatic forces, and hydrophobic forces. Majority of biological processes in the living cell are executed by proteins, and any particular protein function is regulated by numerous other proteins. Thus, knowledge of protein-protein interaction is necessary to understand the biological processes. In this chapter, we explain the widely used yeast two-hybrid assay to identify the protein-interacting partners.
蛋白质-蛋白质相互作用是指两个或多个蛋白质之间的特异性和直接物理接触，这种相互作用涉及氢键、静电力和疏水相互作用力。活细胞中的大多数生物过程都是由蛋白质执行的，而任何特定的蛋白质功能都受到许多其他蛋白质的调节。因此，了解蛋白质-蛋白质相互作用对于理解生物过程是必要的。在本章中，我们解释了广泛使用的酵母双杂交测定法来鉴定蛋白质相互作用的伙伴。
