Department of Plant Pathology, Citrus Research and Education Center, IFAS, University of Florida, Lake Alfred, FL, 33850, USA; Department of Plant Protection, Faculty of Agriculture, Tanta University, 31527, Egypt.
Department of Entomology and Nematology, IFAS, University of Florida, Gainesville, FL 32611, USA.
J Invertebr Pathol. 2023 Sep;200:107972. doi: 10.1016/j.jip.2023.107972. Epub 2023 Jul 17.
The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae) transmits the Gram-negative bacterium 'Candidatus Liberibacter asiaticus' that causes citrus greening disease. While chemical control has been the main management strategy for limiting D. citri, the widespread usage of chemical sprays has decreased the susceptibility of D. citri to most insecticides. Pesticidal proteins produced by the bacterium Bacillus thuringiensis (Bt) are active against a wide variety of insects and provide a more sustainable approach to insect control. Herein, we investigated the impact of 'Ca. L. asiaticus' infection of D. citri on the toxicity of two Bt proteins (Mpp51Aa1 and Cry1Ba1). Proteins were delivered to healthy and 'Ca. L. asiaticus'-infected D. citri via topical feeding application. The LC values of Mpp51Aa1 and Cry1Ba1 were calculated for both nymphs and adults. Additionally, we evaluated the effect of each protein on the survival probability and life span of healthy and 'Ca. L. asiaticus'-infected D. citri. The LC values indicated that adults and nymphs were more susceptible to Mpp51Aa1 than to Cry1Ba1 in both healthy and 'Ca. L. asiaticus'-infected D. citri. 'Ca. L. asiaticus'-infected adults and nymphs were more susceptible to Mpp51Aa1 and Cry1Ba1 than healthy insects, and nymphs were more susceptible to Mpp51Aa1 and Cry1Ba1 than adults. Moreover, we found that Mpp51Aa1 had a greater impact than Cry1Ba1 on the survival and lifespan of adults, and 'Ca. L. asiaticus'-infected insects were more affected by these pesticidal proteins than healthy adults. These results have important implications for the use of pesticidal proteins in D. citri management in Florida and elsewhere given the widespread presence of 'Ca. L. asiaticus' in the D. citri population. In this era of eco-friendly control strategies, Bt-derived pesticidal proteins provide a promising avenue to reducing the application of chemical insecticides for D. citri management.
柑橘木虱，Diaphorina citri Kuwayama（半翅目：丽蚜科）传播革兰氏阴性细菌“亚洲韧皮部杆菌”，导致柑橘黄龙病。虽然化学防治一直是限制柑橘木虱的主要管理策略，但化学喷雾的广泛使用降低了柑橘木虱对大多数杀虫剂的敏感性。由苏云金芽孢杆菌（Bt）产生的杀虫蛋白对多种昆虫有效，为昆虫防治提供了一种更可持续的方法。在此，我们研究了柑橘木虱感染“亚洲韧皮部杆菌”对两种 Bt 蛋白（Mpp51Aa1 和 Cry1Ba1）毒性的影响。通过局部喂食应用将蛋白质递送到健康和“亚洲韧皮部杆菌”感染的柑橘木虱中。计算了 Mpp51Aa1 和 Cry1Ba1 对若虫和成虫的 LC 值。此外，我们评估了每种蛋白质对健康和“亚洲韧皮部杆菌”感染的柑橘木虱的生存概率和寿命的影响。LC 值表明，在健康和“亚洲韧皮部杆菌”感染的柑橘木虱中，成虫和若虫对 Mpp51Aa1 的敏感性均高于 Cry1Ba1。与健康昆虫相比，“亚洲韧皮部杆菌”感染的成虫和若虫对 Mpp51Aa1 和 Cry1Ba1 的敏感性更高，而若虫对 Mpp51Aa1 和 Cry1Ba1 的敏感性高于成虫。此外，我们发现 Mpp51Aa1 对成虫的生存和寿命的影响大于 Cry1Ba1，并且“亚洲韧皮部杆菌”感染的昆虫比健康成虫受这些杀虫蛋白的影响更大。鉴于“亚洲韧皮部杆菌”在柑橘木虱种群中的广泛存在，这些结果对佛罗里达州和其他地区使用杀虫蛋白来管理柑橘木虱具有重要意义。在这个环保控制策略的时代，Bt 衍生的杀虫蛋白为减少化学杀虫剂在柑橘木虱管理中的应用提供了一个有前途的途径。
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