Department of Clinical Biochemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-092 Bydgoszcz, Poland.
Department of Human Biology, Institute of Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Toruń, Poland.
Cell Physiol Biochem. 2023 Jun 19;57(4):200-211. doi: 10.33594/000000638.
BACKGROUND/AIMS: Seminal plasma composition is affected by the physiological state of the prostate, the major male reproductive gland. Semen components, like vitamin C, can modulate sperm function. Vitamin C is an effective scavenger of free radicals and is an essential component of enzymes such as TET proteins involved in the DNA demethylation process. In the present study, a broad range of parameters which may influence the metabolic state of the prostate gland were analysed including blood and prostate tissue vitamin C, epigenetic DNA modifications and 8-oxo-7,8-dihydro-2'-deoxyguanosine in DNA of leukocytes and prostate tissues.
The experimental material were tissue samples from patients with benign prostatic hyperplasia (BPH), normal/marginal prostate tissues from prostate cancer patients, leukocytes from healthy donors, and blood plasma from BPH patients and healthy donors. We applied ultra-performance liquid chromatography methods with mass spectrometry and/or UV detection.
We found an unprecedentedly high level of intracellular vitamin C in all analysed prostatic tissues (benign prostatic hyperplasia and normal, marginal ones), a value much higher than in leukocytes and most human tissues. DNA epigenetic patterns in prostate cells are similar to other soft tissues like the colon, however, its uniqueness is the unprecedentedly high level of 5-(hydroxymethyl)-2'-deoxyuridine and a significant increase in 5-formyl-2'-deoxycytidine value compared to aforementioned tissues. Moreover, the level of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an established marker of oxidative stress, is significantly higher in prostate tissues than in leukocytes and many previously studied soft tissues.
Our results pointed out that prostatic vitamin C (regarded as the main supplier of the vitamin C to seminal plasma) and the DNA modifications (which may be linked to the regeneration of prostate epithelium) may play important role to maintain the prostate health.
背景/目的：精液的组成受到前列腺这个主要男性生殖腺的生理状态的影响。精液成分，如维生素 C，可以调节精子功能。维生素 C 是自由基的有效清除剂，也是 TET 蛋白等参与 DNA 去甲基化过程的酶的必需成分。在本研究中，分析了可能影响前列腺代谢状态的广泛参数，包括血液和前列腺组织中的维生素 C、表观遗传 DNA 修饰以及白细胞和前列腺组织中 DNA 的 8-氧-7,8-二氢-2'-脱氧鸟苷。
实验材料为良性前列腺增生（BPH）患者的组织样本、前列腺癌患者的正常/边缘前列腺组织、健康供者的白细胞以及 BPH 患者和健康供者的血浆。我们应用超高效液相色谱法结合质谱和/或紫外检测法。
我们发现所有分析的前列腺组织（良性前列腺增生和正常、边缘组织）中的细胞内维生素 C 水平异常高，远高于白细胞和大多数人体组织。前列腺细胞的 DNA 表观遗传模式与结肠等其他软组织相似，但它的独特之处在于 5-(羟甲基)-2'-脱氧尿苷的水平异常高，以及 5-甲酰基-2'-脱氧胞苷的显著增加，与上述组织相比。此外，8-氧-7,8-二氢-2'-脱氧鸟苷的水平，一种公认的氧化应激标志物，在前列腺组织中明显高于白细胞和许多先前研究的软组织。
我们的结果表明，前列腺维生素 C（被认为是精液中维生素 C 的主要供应者）和 DNA 修饰（可能与前列腺上皮的再生有关）可能在维持前列腺健康方面发挥重要作用。
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