School of Modern Agriculture, Jiaxing Vocational & Technical College, Jiaxing, 314036, PR China; Department of Civil Engineering, Tongji Zhejiang College, Jiaxing, 314051, PR China.
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai, 200237, PR China.
Environ Res. 2023 Oct 15;235:116594. doi: 10.1016/j.envres.2023.116594. Epub 2023 Jul 17.
As a biological promising wastewater treatment technology, aerobic granular sludge (AGS) technology had been widely studied in sequencing batch reactors (SBRs) for the decades. Presently, the whole processes of its granulation, long-term operation, storage, and reactivation have not been thoroughly evaluated, and also the relationships among microbial diversity, granular size, and characteristics were still not that clear. Hence, they were systematically evaluated in an AGS-SBR in this work. The results demonstrated that Proteobacteria and Bacteroidetes were the dominant phyla, Flavobacterium, Acinetobacter, Azoarcus, and Chryseobacterium were the core genera with discrepant abundances in diverse stages or granular size. Microbial immigration was significant in various stages due to microbial diversity had a line relationship with COD/MLVSS ratio (R = 0.367). However, microbial diversity had no line relationship with granular size (R = 0.001), indicating the microbial diversity in different-sized AGS was similar, although granular size had a line relationship with settleability (R = 0.978). Overall, compared to sludge traits (e.g., sludge size, settleability), COD/MLVSS played a key role on microbial evolution. This study revealed the relationships between granule characteristics and microbial community, and contributed to the future AGS-related studies.
作为一种有前景的生物废水处理技术，好氧颗粒污泥 (AGS) 技术在序批式反应器 (SBR) 中已经得到了几十年的广泛研究。目前，AGS 的形成、长期运行、储存和再激活的整个过程尚未得到彻底评估，微生物多样性、颗粒大小和特性之间的关系也不明确。因此，本工作在 AGS-SBR 中对其进行了系统评估。结果表明，变形菌门和拟杆菌门是主要的门，黄杆菌属、不动杆菌属、节杆菌属和色杆菌属是核心属，在不同阶段或颗粒大小上的丰度存在差异。由于微生物多样性与 COD/MLVSS 比值呈线性关系 (R=0.367)，因此在不同阶段微生物的迁移是显著的。然而，微生物多样性与颗粒大小没有线性关系 (R=0.001)，表明不同粒径 AGS 中的微生物多样性相似，尽管颗粒大小与沉降性呈线性关系 (R=0.978)。总的来说，与污泥特性（如污泥粒径、沉降性）相比，COD/MLVSS 对微生物进化起着关键作用。本研究揭示了颗粒特性与微生物群落之间的关系，为未来的 AGS 相关研究提供了参考。
