一种用于评估类视黄醇对无毛大鼠表皮中诱导型鸟氨酸脱羧酶活性抑制作用的快速简便测试系统。

A rapid and simple test system for the evaluation of the inhibitory activity of retinoids on induced ornithine decarboxylase activity in the hairless rat epidermis.

作者信息

Bouclier M, Shroot B, Eustache J, Hensby C N

出版信息

J Pharmacol Methods. 1986 Sep;16(2):151-60. doi: 10.1016/0160-5402(86)90020-3.

Abstract

In mouse skin, antiproliferative agents including retinoids inhibit induction of ornithine decarboxylase activity by a variety of hyperproliferative stimuli. In the hairless rat skin, ornithine decarboxylase activity was induced by ten successive strippings with cellotape and by topical application of 12-O-tetradecanoyl-phorbol-13-acetate. Topical application of all trans-retinoic acid (25 nmol/cm2) immediately after the tape stripping of the skin significantly inhibited the induction of ornithine decarboxylase activity at all time points measured. The inhibition by all trans-retinoic acid of ornithine decarboxylase induced by cellotape stripping was dose dependent as was found to be the case for arotinoid, retinol, Ro-10-1670, motretinid, 13-cis-retinoic acid, etretinate, and vitamin A. Oral administration of all trans-retinoic acid also inhibited the ornithine decarboxylase activity induced by cellotape stripping. We propose the assay of ornithine decarboxylase activity in the hairless rat epidermis after tape stripping for a rapid evaluation of new retinoids.

摘要

在小鼠皮肤中，包括类视黄醇在内的抗增殖剂可抑制多种过度增殖刺激物诱导的鸟氨酸脱羧酶活性。在无毛大鼠皮肤中，用透明胶带连续剥离10次以及局部应用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯均可诱导鸟氨酸脱羧酶活性。在皮肤胶带剥离后立即局部应用全反式维甲酸（25 nmol/cm²），在所有测量时间点均显著抑制了鸟氨酸脱羧酶活性的诱导。全反式维甲酸对胶带剥离诱导的鸟氨酸脱羧酶的抑制作用呈剂量依赖性，阿罗替诺、视黄醇、Ro - 10 - 1670、莫替维德、13 - 顺式维甲酸、依曲替酯和维生素A的情况也是如此。口服全反式维甲酸也抑制了胶带剥离诱导的鸟氨酸脱羧酶活性。我们建议通过检测胶带剥离后无毛大鼠表皮中的鸟氨酸脱羧酶活性来快速评估新型类视黄醇。