is involved in regulating the tolerance of rice to copper stress.

INTRODUCTION

Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in metal absorption, transport and accumulation in plants. However, so far, only several plant HIPPs have been functionally analyzed. In this study, a novel HIPP member , which was involved in the tolerance to copper (Cu) was functionally characterized.

METHODS

In this study, qRT-PCR, Yeast transgenic technology, Plant transgenic technology, ICP-MS and so on were used for research.

RESULTS

OsHIPP17 protein was targeted to the nucleus. The Cu concentration reached 0.45 mg/g dry weight due to the overexpression of in yeast cells. Meanwhile, the overexpression of resulted in the compromised growth of () under Cu stress. The root length of mutant lines was also significantly reduced by 16.74- 24.36% under 25 mM Cu stress. The roots of rice mutant showed increased Cu concentration by 7.25%-23.32%. Meanwhile, knockout of OsHIPP17 decreased the expression levels of , , or , and increased the expression levels of or . Antioxidant enzyme activity was also reduced in rice due to the knockout of . Moreover, the expression levels of cytokinin-related genes in plants under Cu stress were also affected by overexpression or knockout of .

DISCUSSION

These results implied that might play a role in plant Cu toxic response by affecting the expression of Cu transport genes or cytokinin-related genes. Simultaneously, our work may shed light on the underlying mechanism of how heavy metals affect the plant growth and provide a novel rice genetic source for phytoremediation of heavy metal-contaminated soil.