Allsman Lindy A, Bellinger Marschal A, Huang Vivian, Duong Matthew, Contreras Alondra, Romero Andrea N, Verboonen Benjamin, Sidhu Sukhmani, Zhang Xiaoguo, Steinkraus Holly, Uyehara Aimee N, Martinez Stephanie E, Sinclair Rosalie M, Soriano Gabriela Salazar, Diep Beatrice, Byrd V Dawson, Noriega Alexander, Drakakaki Georgia, Sylvester Anne W, Rasmussen Carolyn G
Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
Department of Molecular Biology, University of Wyoming, Laramie, WY, United States.
Front Plant Sci. 2023 Jul 7;14:1204889. doi: 10.3389/fpls.2023.1204889. eCollection 2023.
During proliferative plant cell division, the new cell wall, called the cell plate, is first built in the middle of the cell and then expands outward to complete cytokinesis. This dynamic process requires coordinated movement and arrangement of the cytoskeleton and organelles.
Here we use live-cell markers to track the dynamic reorganization of microtubules, nuclei, endoplasmic reticulum, and endomembrane compartments during division and the formation of the cell plate in maize leaf epidermal cells.
The microtubule plus-end localized protein END BINDING1 (EB1) highlighted increasing microtubule dynamicity during mitosis to support rapid changes in microtubule structures. The localization of the cell-plate specific syntaxin KNOLLE, several RAB-GTPases, as well as two plasma membrane localized proteins was assessed after treatment with the cytokinesis-specific callose-deposition inhibitor Endosidin7 (ES7) and the microtubule-disrupting herbicide chlorpropham (CIPC). While ES7 caused cell plate defects in , it did not alter callose accumulation, or disrupt cell plate formation in maize. In contrast, CIPC treatment of maize epidermal cells occasionally produced irregular cell plates that split or fragmented, but did not otherwise disrupt the accumulation of cell-plate localized proteins.
Together, these markers provide a robust suite of tools to examine subcellular trafficking and organellar organization during mitosis and cell plate formation in maize.
在植物细胞增殖分裂过程中，新的细胞壁即细胞板，首先在细胞中部形成，然后向外扩展以完成胞质分裂。这一动态过程需要细胞骨架和细胞器的协同运动与排列。
在此，我们使用活细胞标记物来追踪玉米叶片表皮细胞分裂和细胞板形成过程中微管、细胞核、内质网和内膜区室的动态重组。
微管正端定位蛋白END BINDING1（EB1）突显了有丝分裂期间微管动态性的增加，以支持微管结构的快速变化。在用胞质分裂特异性胼胝质沉积抑制剂Endosidin7（ES7）和破坏微管的除草剂氯苯胺灵（CIPC）处理后，评估了细胞板特异性 syntaxin KNOLLE、几种RAB - GTP酶以及两种质膜定位蛋白的定位。虽然ES7在[具体情况未明确说明]中导致细胞板缺陷，但它并未改变胼胝质积累，也未破坏玉米中的细胞板形成。相比之下，用CIPC处理玉米表皮细胞偶尔会产生分裂或破碎成不规则的细胞板，但并未以其他方式破坏细胞板定位蛋白的积累。
总之，这些标记物提供了一套强大的工具，用于研究玉米有丝分裂和细胞板形成过程中的亚细胞运输和细胞器组织。
