Instituto Nacional do Câncer, Coordenação de Pesquisa, Rio de Janeiro, RJ, Brazil.
Adv Exp Med Biol. 2023;1429:59-71. doi: 10.1007/978-3-031-33325-5_4.
The use of CRISPR as a genetic editing tool modified the oncology field from its basic to applied research for opening a simple, fast, and cheaper way to manipulate the genome. This chapter reviews some of the major uses of this technique for in vitro- and in vivo-based biological screenings, for cellular and animal model generation, and new derivative tools applied to cancer research. CRISPR has opened new frontiers increasing the knowledge about cancer, pointing to new solutions to overcome several challenges to better understand the disease and design better treatments.
CRISPR 作为一种基因编辑工具，从基础研究到应用研究改变了肿瘤学领域，为操纵基因组提供了一种简单、快速和更经济的方法。本章综述了该技术在基于体外和体内的生物筛选、细胞和动物模型生成，以及应用于癌症研究的新衍生工具中的一些主要用途。CRISPR 开辟了新的前沿领域，增加了对癌症的认识，为克服理解疾病和设计更好治疗方法的多个挑战提供了新的解决方案。
