Koepsel R R, Khan S A
Science. 1986 Sep 19;233(4770):1316-8. doi: 10.1126/science.3749879.
DNA bending has been suggested to play a role in the regulation of gene expression, initiation of DNA replication, DNA packaging, and the recognition of specific DNA sequences by proteins. It has recently been demonstrated that DNA bending can be sequence-directed. Bent DNA has also been observed as a consequence of sequence-specific binding of proteins to DNA. In this report DNA of plasmid pT181 is shown to contain a bend at the replication origin. Furthermore, this bend is enhanced by the binding of the pT181 replication initiator protein, RepC, to the origin.
有人提出DNA弯曲在基因表达调控、DNA复制起始、DNA包装以及蛋白质对特定DNA序列的识别中发挥作用。最近已证明DNA弯曲可以由序列引导。由于蛋白质与DNA的序列特异性结合，也观察到了弯曲的DNA。在本报告中，质粒pT181的DNA在复制起点处显示有一个弯曲。此外，pT181复制起始蛋白RepC与起点的结合增强了这种弯曲。
