Liu Jiuxi, Cai Jiapei, Fan Peng, Dong Xue, Zhang Naisheng, Tai Jiandong, Cao Yongguo
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 130062 Changchun, People's Republic of China.
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, 510530 Guangzhou, People's Republic of China.
Food Funct. 2023 Aug 14;14(16):7506-7519. doi: 10.1039/d3fo01929b.
Dysbiosis causes continuous progress of inflammatory bowel disease (IBD). Herein, we aim to explore whether Salidroside (Sal), which is a major glycoside extracted from L., could ameliorate dextran sulfate sodium (DSS)-induced colitis by modulating the microbiota. Results showed that oral treatment with 15 mg kg of Sal inhibited DSS-induced colitis in mice as evidenced by colon length, histological analysis, disease activity index (DAI) score, and the proportion and number of macrophages in the intestine. The gut microbiota of colitic mice was also partly restored by Sal. A fecal microbiota transplantation (FMT) study was designed to verify the causality. Compared with DSS-treated mice, FM from the Sal-treated donor mice significantly mitigated the symptoms of colitic mice, including reducing the DAI score, alleviating tissue damage, boosting the expression of mucin protein (mucin-2) and tight junction (TJ) proteins (occludin and zonula occludens-1 (ZO-1), and decreasing M1 macrophages in the gut. It was found that both Sal and FMT affected the structure and abundance of the gut microbiota as reflected by the decreased relative abundance of , , and the increased relative abundance of at the genus level. Moreover, the anti-inflammatory effect of Sal disappeared when the gut microbiota was depleted by antibiotics, demonstrating that Sal alleviated the intestinal inflammation in a gut microbiota-dependent manner. Thus, Sal could be a remarkable candidate as a functional food for colitis.
肠道菌群失调导致炎症性肠病（IBD）持续进展。在此，我们旨在探究红景天苷（Sal），一种从红景天中提取的主要糖苷，是否能通过调节微生物群来改善葡聚糖硫酸钠（DSS）诱导的结肠炎。结果显示，口服15 mg/kg的Sal可抑制DSS诱导的小鼠结肠炎，这通过结肠长度、组织学分析、疾病活动指数（DAI）评分以及肠道中巨噬细胞的比例和数量得以证明。Sal还部分恢复了结肠炎小鼠的肠道微生物群。设计了一项粪便微生物群移植（FMT）研究以验证因果关系。与DSS处理的小鼠相比，来自Sal处理供体小鼠的粪便微生物群显著减轻了结肠炎小鼠的症状，包括降低DAI评分、减轻组织损伤、促进粘蛋白（mucin - 2）和紧密连接（TJ）蛋白（闭合蛋白和闭合小带蛋白1（ZO - 1））的表达，以及减少肠道中的M1巨噬细胞。研究发现，Sal和FMT均影响肠道微生物群的结构和丰度，这在属水平上表现为[具体菌属名称1]、[具体菌属名称2]、[具体菌属名称3]相对丰度降低以及[具体菌属名称4]相对丰度增加。此外，当用抗生素耗尽肠道微生物群时，Sal的抗炎作用消失，这表明Sal以肠道微生物群依赖的方式减轻肠道炎症。因此，Sal可能是一种用于结肠炎的功能性食品的优秀候选物。
