分析 E-钙黏蛋白（CDH1）基因多态性及其与孟加拉国女性宫颈癌风险的关系。

Analysis of E-cadherin (CDH1) Gene Polymorphism and Its Association with Cervical Cancer Risk in Bangladeshi Women.

机构信息

Pharmacy Discipline, Khulna University, Khulna, Bangladesh.

Department of Pharmacy, Dhaka International University, Dhaka, Bangladesh.

出版信息

Asian Pac J Cancer Prev. 2023 Jul 1;24(7):2361-2368. doi: 10.31557/APJCP.2023.24.7.2361.

DOI:10.31557/APJCP.2023.24.7.2361

PMID:37505767

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10676483/

Abstract

BACKGROUND

E-cadherin (CDH1), a tumor suppressor gene, encodes a transmembrane glycoprotein that helps in maintaining squamous epithelium integrity of the cervix. We aimed to investigate the association between -160C/A genetic polymorphism in CDH1 and the risk of cervical cancer in Bangladeshi females.

METHOD

The present case-control study included 117 cervical cancer cases and 147 age-matched controls. The genomic DNA was extracted from peripheral blood and genotyped by using PCR-RFLP analysis.

RESULTS

Genotyping results demonstrated that the occurrences of normal homozygous (-160C/C), heterozygous (-160C/A) and variant homozygous (-160A/A) genotypes were 64.10, 27.35 and 8.55% in cases, and 77.55, 19.73 and 2.72% in controls, respectively. Compared to normal C/C genotype, variant A/A and combined (C/A+A/A) or 'any A' genotypes exhibited 3.80-fold (95% CI=1.150-12.561, P=0.029) and 1.93-fold (95% CI=1.126-3.323, P=0.017) increased risk of cervical cancer development. The -160C allele was found to be positively linked to cervical cancer incidence and raised the risk by 1.81-fold (OR= 1.814, 95% CI=1.152-2.857, p=0.01). Moreover, women carrying -160A/A variant homozygosity along with an early marital history (<18 years) were more susceptible to cervical cancer development (χ2 =6.605, p=0.037).

CONCLUSION

The study suggests that the (A/A) and combined (C/A +A/A) genotypes are associated with greater risk of cervical cancer in Bangladeshi women.

摘要

背景

E-钙黏蛋白（CDH1）是一种肿瘤抑制基因，编码一种跨膜糖蛋白，有助于维持宫颈鳞状上皮的完整性。我们旨在研究 CDH1 基因-160C/A 遗传多态性与孟加拉国女性宫颈癌风险之间的关联。

方法

本病例对照研究包括 117 例宫颈癌病例和 147 例年龄匹配的对照。从外周血中提取基因组 DNA，并通过 PCR-RFLP 分析进行基因分型。

结果

基因分型结果表明，病例组正常纯合子（-160C/C）、杂合子（-160C/A）和变异纯合子（-160A/A）的发生率分别为 64.10%、27.35%和 8.55%，对照组分别为 77.55%、19.73%和 2.72%。与正常 C/C 基因型相比，变异 A/A 基因型以及合并（C/A+A/A）或“任何 A”基因型显示出 3.80 倍（95%CI=1.150-12.561，P=0.029）和 1.93 倍（95%CI=1.126-3.323，P=0.017）的宫颈癌发展风险增加。-160C 等位基因与宫颈癌的发生呈正相关，风险增加 1.81 倍（OR=1.814，95%CI=1.152-2.857，p=0.01）。此外，携带 -160A/A 变异纯合子且早婚史（<18 岁）的女性更容易发生宫颈癌（χ2=6.605，p=0.037）。