Feng Wen, Zhou Lei, Zhao Pengju, Du Heng, Diao Chenguang, Zhang Yu, Liu Zhen, Jin Wenjiao, Yu Jian, Han Jianlin, Okoth Edward, Mrode Raphael, Liu Jian-Feng
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture; Frontiers Science Center for Molecular Design Breeding (MOE), College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Shenzhen Kingsino Technology Co., Ltd., Shenzhen 518107, China.
Biology (Basel). 2023 Jul 14;12(7):1001. doi: 10.3390/biology12071001.
As warthogs () have innate immunity against African swine fever (ASF), it is critical to understand the evolutionary novelty of warthogs to explain their specific ASF resistance.
Here, we present two completed new genomes of one warthog and one Kenyan domestic pig as fundamental genomic references to elucidate the genetic mechanisms of ASF tolerance.
Multiple genomic variations, including gene losses, independent contraction, and the expansion of specific gene families, likely molded the warthog genome to adapt to the environment. Importantly, the analysis of the presence and absence of genomic sequences revealed that the DNA sequence of the warthog genome had an absence of the gene lactate dehydrogenase B () on chromosome 2 compared with the reference genome. The overexpression and siRNA of inhibited the replication of the African swine fever virus. Combined with large-scale sequencing data from 42 pigs worldwide, the contraction and expansion of tripartite motif-containing (TRIM) gene families revealed that TRIM family genes in the warthog genome are potentially responsible for its tolerance to ASF.
Our results will help improve the understanding of genetic resistance to ASF in pigs.
由于疣猪对非洲猪瘟（ASF）具有先天免疫力，了解疣猪的进化新奇性对于解释其对ASF的特异性抗性至关重要。
在此，我们展示了一头疣猪和一头肯尼亚家猪的两个完整新基因组，作为阐明ASF耐受性遗传机制的基础基因组参考。
多种基因组变异，包括基因缺失、独立收缩以及特定基因家族的扩张，可能塑造了疣猪基因组以适应环境。重要的是，对基因组序列存在与否的分析表明，与参考基因组相比，疣猪基因组的DNA序列在2号染色体上缺少乳酸脱氢酶B（）基因。的过表达和siRNA抑制了非洲猪瘟病毒的复制。结合来自全球42头猪的大规模测序数据，含三联基序（TRIM）基因家族的收缩和扩张表明，疣猪基因组中的TRIM家族基因可能是其对ASF耐受性的原因。
我们的结果将有助于提高对猪对ASF遗传抗性的理解。
