对真菌的吩嗪-1-羧酸抗真菌机制研究。

Antifungal Mechanism of Phenazine-1-Carboxylic Acid against .

机构信息

Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.

College of Tea, Guizhou University, Guiyang 550025, China.

出版信息

Int J Mol Sci. 2023 Jul 10;24(14):11274. doi: 10.3390/ijms241411274.

DOI:10.3390/ijms241411274

PMID:37511033

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10379350/

Abstract

sp. is an important class of plant pathogenic fungi that can infect a variety of crops. We have proved the pathogenicity of on bayberry leaves and caused bayberry blight. Phenazine-1-carboxylic acid (PCA) has the characteristics of high efficiency, low toxicity, and environmental friendliness, which can prevent fungal diseases on a variety of crops. In this study, the effect of PCA on the morphological, physiological, and molecular characteristics of has been investigated, and the potential antifungal mechanism of PCA against was also explored. We applied PCA on in vitro and in vivo to determine its inhibitory effect on PCA. It was found that PCA was highly efficient against with EC around 2.32 μg/mL, and the in vivo effect was 57% at 14 μg/mL. The mechanism of PCA was preliminarily explored by transcriptomics technology. The results showed that after the treatment of PCA, 3613 differential genes were found, focusing on redox processes and various metabolic pathways. In addition, it can also cause mycelial development malformation, damage cell membranes, reduce mitochondrial membrane potential, and increase ROS levels. This result expanded the potential agricultural application of PCA and revealed the possible mechanism against .

摘要

sp. 是一类重要的植物病原真菌，可侵染多种作物。我们已经证明对杨梅叶片具有致病性，并引起杨梅疫病。吩嗪-1-羧酸（PCA）具有高效、低毒、环保的特点，可防治多种作物的真菌病害。本研究探讨了 PCA 对的形态、生理和分子特性的影响，并探索了 PCA 对的潜在抑菌机制。我们在体外和体内应用 PCA 来确定其对 PCA 的抑制作用。结果表明，PCA 对具有高效性，EC₅₀ 值约为 2.32 μg/mL，在 14 μg/mL 时的体内效果为 57%。通过转录组学技术初步探讨了 PCA 的作用机制。结果表明，经 PCA 处理后，发现 3613 个差异基因，主要集中在氧化还原过程和各种代谢途径。此外，它还可以导致菌丝发育畸形、破坏细胞膜、降低线粒体膜电位和增加 ROS 水平。这一结果扩展了 PCA 在农业上的潜在应用，并揭示了其可能的抑菌机制。