中国核桃枝枯病相关病菌的分子鉴定与特性分析

Molecular Identification and Characterization of Associated with Walnut Branch Blight Disease in China.

作者信息

Ma Ting, Yang Chengde, Cai Fengfeng, Osei Richard

机构信息

Biocontrol Engineering Laboratory of Crop Diseases and Pests, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.

出版信息

Pathogens. 2023 Jul 24;12(7):970. doi: 10.3390/pathogens12070970.

DOI:10.3390/pathogens12070970

PMID:37513816

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10384706/

Abstract

In October 2020, samples of walnut branch blight were collected from Longnan. Pathogens were isolated and identified based on morphological and molecular features, and their characteristics were analyzed by pathogenicity. Pathogenicity testing revealed that seven strains (LN-1, LN-3, LN-6, LN-19, LN-27, QY3-1, and QY9-1) induced symptoms of walnut branch blight that were consistent with those observed in the field after inoculation. Furthermore, some -type conidia and spherical chlamydospores were visible indicating that they were spp. A molecular characterization including sequence and phylogenetic analysis of the , , , and gene regions revealed that LN-1 and LN-19 belonged to , LN-3 and LN-6 to , LN-27 to , and QY3-1 and QY9-1 to . This is the first time that -, , and -caused walnut branch blight has been reported in China.

摘要

2020年10月，从陇南采集了核桃枝枯病样本。基于形态学和分子特征对病原菌进行分离和鉴定，并通过致病性分析其特性。致病性测试表明，7个菌株（LN-1、LN-3、LN-6、LN-19、LN-27、QY3-1和QY9-1）诱发的核桃枝枯病症状与接种后田间观察到的症状一致。此外，可见一些类型的分生孢子和球形厚垣孢子，表明它们是 spp.。对、、、和基因区域进行的包括序列和系统发育分析在内的分子特征鉴定表明，LN-1和LN-19属于，LN-3和LN-6属于，LN-27属于，QY3-1和QY9-1属于。这是中国首次报道由、和引起的核桃枝枯病。