Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
Gene Vantage, 53 Kyalami Boulevard, Kyalami Business Park, Johannesburg 1684, South Africa.
Viruses. 2023 Jun 29;15(7):1474. doi: 10.3390/v15071474.
South Africa is associated with a centuries-old viticultural industry, accompanied by a diverse range of wine and table grape cultivars and an extensive history of pervasive introductions of vine material and associated viruses. The D2 collection in Stellenbosch represents the most comprehensive collection of species, hybrids, and cultivars in South Africa. We collected leaf petiole material from 229 accessions from this collection. Our metaviromic analyses revealed a total of 406 complete/near complete genomes of various betaflexiviruses. Among these, we identified the presence of grapevine rupestris stem pitting-associated virus and grapevine viruses A, B, E, F, H (GVH), I (GVI), and M (GVM). Notably, this study marks the first report of GVH, GVI, and GVM in South Africa, which were confirmed via RT-PCR. This research significantly contributes to our understanding of viral diversity and introductions in South African viticulture and emphasizes the need for vigilant monitoring and management of viral infections. Our findings lay the groundwork for strategies that mitigate the impact of viruses on South Africa's wine industry, which generates an annual revenue of approximately 500 million USD.
南非拥有悠久的葡萄栽培历史，拥有多样化的葡萄酒和鲜食葡萄品种，以及广泛的葡萄材料和相关病毒的广泛引入历史。斯坦陵布什的 D2 收藏是南非最全面的品种、杂交品种和栽培品种收藏。我们从该收藏的 229 个品种中采集了叶片叶柄材料。我们的 metaviromic 分析共揭示了 406 种不同的贝塔 flexiviruses 的完整/近完整基因组。其中，我们发现了葡萄岩纹病茎陷点相关病毒和葡萄病毒 A、B、E、F、H（GVH）、I（GVI）和 M（GVM）的存在。值得注意的是，本研究首次在南非报告了 GVH、GVI 和 GVM，通过 RT-PCR 得到了证实。这项研究极大地促进了我们对南非葡萄栽培中病毒多样性和引入的理解，并强调需要对病毒感染进行警惕监测和管理。我们的研究结果为减轻病毒对南非葡萄酒行业的影响的战略奠定了基础，该行业每年的收入约为 5 亿美元。
