School of Engineering and Sciences, Tecnologico de Monterrey, Campus Queretaro, Queretaro 76130, Mexico.
Departments of Computer Science and Evolution and Ecology, University of California-Davis, Davis, CA 95616, USA.
Viruses. 2023 Jul 16;15(7):1561. doi: 10.3390/v15071561.
This is the first viral metagenomic analysis of grapevine conducted in Mexico. During the summer of 2021, 48 plants displaying virus-like symptoms were sampled in Queretaro, an important grapevine-producing area of Mexico, and analyzed for the presence of viruses via high-throughput sequencing (HTS). The results of HTS were verified by real-time RT-PCR following a standardized testing scheme (Protocol 2010). Fourteen different viruses were identified, including grapevine asteroid mosaic-associated virus (GAMaV), grapevine Cabernet Sauvignon reovirus (GCSV), grapevine fanleaf virus (GFLV), grapevine fleck virus (GFkV), grapevine Pinot gris virus (GPGV), grapevine red globe virus (GRGV), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine rupestris vein feathering virus (GRVFV), grapevine Syrah virus 1 (GSyV-1), grapevine virus B (GVB), and grapevine leafroll-associated viruses 1, 2, 3, 4 (GLRaV1, 2, 3, 4). Additionally, divergent variants of GLRaV4 and GFkV, and a novel -like virus were discovered. This is the first report of GAMaV, GCSV, GLRaV4, GPGV, GRGV, GRVFV, and GSyV-1 infecting grapevines in Mexico; the impact of these pathogens on production is unknown.
这是首次在墨西哥对葡萄进行病毒宏基因组分析。2021 年夏季，在墨西哥重要的葡萄种植区——克雷塔罗州，采集了 48 株表现病毒样症状的植株，通过高通量测序（HTS）分析病毒的存在。HTS 的结果通过标准化测试方案（方案 2010）下的实时 RT-PCR 进行验证。共鉴定出 14 种不同的病毒，包括葡萄斑纹镶嵌病毒（GAMaV）、葡萄赤霞珠呼肠孤病毒（GCSV）、葡萄扇叶病毒（GFLV）、葡萄斑点病毒（GFkV）、葡萄灰葡萄孢病毒（GPGV）、葡萄红地球病毒（GRGV）、葡萄卷叶病毒（GRSPaV）、葡萄卷叶伴随病毒 1、2、3、4（GLRaV1、2、3、4）、葡萄茎痘伴随病毒（GRVFV）、葡萄 Syrah 病毒 1（GSyV-1）、葡萄病毒 B（GVB）以及 GLRaV4 和 GFkV 的分化变体，还发现了一种新型类似病毒。这是墨西哥首次报道 GAMaV、GCSV、GLRaV4、GPGV、GRGV、GRVFV 和 GSyV-1 感染葡萄；这些病原体对生产的影响尚不清楚。
