Espey L L, Miller D H, Margolius H S
Am J Physiol. 1986 Sep;251(3 Pt 1):E362-5. doi: 10.1152/ajpendo.1986.251.3.E362.
Ovarian kinin-generating capacity was determined during induced ovulation in immature Wistar rats. The onset of ovulation was monitored by counting the number of ova in the oviducts at 2-h intervals after the administration of human chorionic gonadotropin (hCG). Ova began to appear in significant numbers at 14 h after hCG, with an average of 7.6 +/- 2.3 ova/rat. By 16 h after hCG, the oviducts contained 32.7 +/- 4.1 ova/rat. The ovaries from each group of animals were homogenized in phosphate-buffered saline, and extracts of this tissue were incubated for 200 min to allow the generation of kinins from endogenous kininogen. The amount of kinin generated by this procedure was measured by radioimmunoassay. At 0 h (i.e., just before the administration of hCG), the ovaries contained 5.90 +/- 0.60 pg kinin/micrograms protein per 200 min in the ovarian extract. By 4 h after hCG, the kinins increased significantly (P less than 0.05) to 13.16 +/- 3.61 pg kinin/micrograms protein. The kinins progressively increased (P less than 0.001) to 67.88 +/- 23.26 pg kinin/micrograms protein by 16 h after hCG. Indomethacin and cycloheximide significantly inhibited both kinin-generating activity and ovulation. These data suggest that kinin-forming activity and kinins may have a role in the ovulatory process of mammals.
在未成熟的Wistar大鼠诱导排卵过程中测定卵巢激肽生成能力。通过在注射人绒毛膜促性腺激素（hCG）后每隔2小时计数输卵管中的卵子数量来监测排卵开始时间。hCG注射后14小时开始出现大量卵子，平均每只大鼠有7.6±2.3个卵子。hCG注射后16小时，输卵管中每只大鼠含有32.7±4.1个卵子。将每组动物的卵巢在磷酸盐缓冲盐水中匀浆，将该组织提取物孵育200分钟，以使内源性激肽原生成激肽。通过放射免疫测定法测量该过程产生的激肽量。在0小时（即，在注射hCG之前），卵巢提取物中每200分钟每微克蛋白质含有5.90±0.60 pg激肽。hCG注射后4小时，激肽显著增加（P<0.05）至13.16±3.61 pg激肽/微克蛋白质。到hCG注射后16小时，激肽逐渐增加（P<0.001）至67.88±23.26 pg激肽/微克蛋白质。吲哚美辛和环己酰亚胺显著抑制激肽生成活性和排卵。这些数据表明激肽形成活性和激肽可能在哺乳动物的排卵过程中起作用。
