National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
Pestic Biochem Physiol. 2023 Aug;194:105494. doi: 10.1016/j.pestbp.2023.105494. Epub 2023 Jun 10.
The Tobamovirus helicase plays an important role in virus proliferation and host interaction. They can also be targets for antiviral drugs. Tobacco mosaic virus (TMV) is well controlled by ningnanmycin (NNM), but whether it acts on other virus helicases of Tobamovirus virus is not clear. In this study, we expressed and purified several Tobamovirus virus helicase proteins and analyzed the three-dimensional structures of several Tobamovirus virus helicases. In addition, the binding of Tobamovirus helicase to NNM was also studied. The docking study reveals the interaction between NNM and Tobamovirus virus helicase. Microscale Thermophoresis (MST) experiments have shown that NNM binds to Tobamovirus helicase with a dissociation constant of 4.64-12.63 μM. Therefore, these data are of great significance for the design and synthesis of new effective anti-plant virus drugs.
烟草花叶病毒（TMV）的运动蛋白在病毒增殖和宿主相互作用中发挥着重要作用，它们也是抗病毒药物的作用靶点。宁南霉素（NNM）对烟草花叶病毒有很好的控制作用，但它是否作用于其他烟草花叶病毒的运动蛋白还不清楚。本研究表达并纯化了几种烟草花叶病毒运动蛋白，并分析了几种烟草花叶病毒运动蛋白的三维结构。此外，还研究了烟草花叶病毒运动蛋白与宁南霉素的结合。对接研究揭示了宁南霉素与烟草花叶病毒运动蛋白的相互作用。微量热泳动（MST）实验表明，宁南霉素与烟草花叶病毒运动蛋白的解离常数为 4.64-12.63 μM。因此，这些数据对于设计和合成新型有效的抗植物病毒药物具有重要意义。
