Han Lingshu, Hao Pengfei, Wang Wenpei, Wu Yanglei, Ruan Shuchao, Gao Chuang, Tian Wanrong, Tian Ye, Li Xiaonan, Wang Luo, Zhang Weijie, Wang Heng, Chang Yaqing, Ding Jun
Key Laboratory of Northern Aquatic Germplasm Resources and Genetic Breeding in Liaoning Province, Dalian 116023, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning, 116023, China; School of Marine Sciences, Ningbo University, Ningbo 315211, China.
Key Laboratory of Northern Aquatic Germplasm Resources and Genetic Breeding in Liaoning Province, Dalian 116023, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, Liaoning, 116023, China.
Sci Total Environ. 2023 Nov 25;901:165846. doi: 10.1016/j.scitotenv.2023.165846. Epub 2023 Aug 2.
In the context of climate change and extreme high temperature, the commercially important sea urchin Strongylocentrotus intermedius suffers high mortality during summer in Northern China. How sea urchins respond to high temperatures is of great concern to academia and industry. How to understand the heat tolerance of sea urchin from the whole transcriptome level. In this study, the heat-resistant S. intermedius bred by our team and its control group were used as the research objects, then we applied whole-transcriptome RNA sequencing to detect differentially expressed mRNAs, microRNAs, long noncoding RNAs that respond to heat stress in the heat-resistant and control S. intermedius. A competitive endogenous RNA (ceRNA) regulatory network was constructed with predicted pairs of differentially expressed mRNAs and noncoding RNAs and revealed the molecular regulatory mechanisms in S. intermedius responding to heat stress. A functional analysis suggested that the ceRNAs were involved in basal metabolism, calcium ion transport, endoplasmic reticulum stress, and apoptosis. This is the whole-transcriptomic analysis of S. intermedius under heat stress to propose ceRNA networks that will provide a basis for studying the potential functions of long noncoding RNAs and miRNAs in the heat stress response in S. intermedius and provide a theoretical basis for the study of the molecular mechanism of sea urchins in response to environmental changes.
在气候变化和极端高温的背景下，具有重要商业价值的中间球海胆在中国北方夏季死亡率很高。海胆如何应对高温是学术界和产业界极为关注的问题。如何从全转录组水平理解海胆的耐热性。在本研究中，以本团队培育的耐热中间球海胆及其对照组为研究对象，应用全转录组RNA测序检测耐热和对照中间球海胆中对热应激有反应的差异表达mRNA、microRNA、长链非编码RNA。构建了一个竞争性内源RNA（ceRNA）调控网络，该网络由预测的差异表达mRNA和非编码RNA对组成，揭示了中间球海胆对热应激的分子调控机制。功能分析表明，ceRNA参与基础代谢、钙离子转运、内质网应激和细胞凋亡。这是对热应激下中间球海胆进行的全转录组分析，提出的ceRNA网络将为研究长链非编码RNA和miRNA在中间球海胆热应激反应中的潜在功能提供依据，并为研究海胆响应环境变化的分子机制提供理论基础。
