Jump D B, Tao T Y, Towle H C, Oppenheimer J H
Endocrinology. 1986 May;118(5):1892-6. doi: 10.1210/endo-118-5-1892.
The messenger RNA (mRNA) coding for the rat hepatic protein spot 14(S14) (mol wt 17,000; pI 4.9) is rapidly responsive to T3 and carbohydrate administration in the adult animal. In 15-day-old suckling rats, the level of expression is 200-fold less than in 60-day-old chow-fed animals. However, the relative transcriptional activity of the S14 gene is only 30% higher in the 60-day-old rat than in the 15-day-old animal. Although T3 induces a 100-fold increase in mRNAS14 in 15-day-old animals, there was only a 17% increase in the S14 gene transcriptional activity. These results indicate that the hormonal and developmental regulation of the S14 gene in liver appears to be predominantly directed at the posttranscriptional level.
编码大鼠肝脏蛋白斑点14（S14）（分子量17,000；等电点4.9）的信使核糖核酸（mRNA）在成年动物中对三碘甲状腺原氨酸（T3）和碳水化合物给药反应迅速。在15日龄的乳鼠中，其表达水平比60日龄以普通饲料喂养的动物低200倍。然而，S14基因的相对转录活性在60日龄大鼠中仅比15日龄动物高30%。尽管T3可使15日龄动物的mRNA S14增加100倍，但S14基因的转录活性仅增加17%。这些结果表明，肝脏中S14基因的激素调节和发育调节似乎主要针对转录后水平。
