红景天苷通过 PI3K/AKT 通路抑制前列腺癌细胞的增殖和迁移。

Salidroside suppresses proliferation and migration in prostate cancer via the PI3K/AKT pathway.

机构信息

Department of combine traditional Chinese and Western Medicine, Huanggang Central Hospital, Huanggang, Hubei, China.

Department of Neurology, Huanggang Central Hospital, Huanggang, China.

出版信息

Cancer Biomark. 2023;38(3):321-332. doi: 10.3233/CBM-220454.

DOI:10.3233/CBM-220454

PMID:37545219

Abstract

BACKGROUND

Prostate cancer (PCa) is one of the most common malignancies in men. PCa is difficult to detect in its early stages, and most patients are diagnosed in the middle to late stages. At present, drug therapy for advanced PCa is still insufficient. Some patients develop drug resistance in the later stage of therapy, which leads to tumor recurrence, metastasis and even treatment failure. Therefore, it is crucial to find new and effective drugs to treat prostate cancer.

OBJECTIVE

The aim of this study was to investigate the anti-cancer effect of salidroside, an active ingredient in a traditional Chinese herbal medicine, on PCa.

METHODS

Two human PCa cell lines, PC3 and DU145, were cultured and treated with salidroside. Cell viability and proliferation ability were analyzed through CCK-8 and colony assays, and cell migration ability was detected by Transwell and Scratch assays. RT-PCR and WB were used to detected the expression levels of moleculars related to cell proliferation, apoptosis, migration, and AKT signaling pathway. Forthmore, we performed rescue experiments with agonist to verify the affected signaling pathway.

RESULTS

Salidroside inhibited the proliferation, colony formation, and migration of PCa cells. Meanwhile, apoptosis of PCa cells was enhanced. Moreover, salidroside inhibited PI3K/AKT pathway in PCa cells. The treatment of AKT agonist 740Y-P abrogated the inhibitory effect of salidroside on the PI3K/AKT signaling pathway.

CONCLUSIONS

Our study demonstrated that in PCa cells, salidroside inhibites proliferation and migration and promots apoptosis via inhibiting PI3K/AKT pathway.

摘要

背景

前列腺癌（PCa）是男性最常见的恶性肿瘤之一。PCa 在早期阶段难以检测到，大多数患者在中晚期被诊断出来。目前，晚期 PCa 的药物治疗仍然不足。一些患者在治疗后期会产生耐药性，导致肿瘤复发、转移甚至治疗失败。因此，寻找新的有效药物来治疗前列腺癌至关重要。

目的

本研究旨在探讨传统中药红景天苷对前列腺癌的抗癌作用。

方法

培养两种人前列腺癌细胞系 PC3 和 DU145，并使用红景天苷进行处理。通过 CCK-8 和集落实验分析细胞活力和增殖能力，通过 Transwell 和划痕实验检测细胞迁移能力。通过 RT-PCR 和 WB 检测与细胞增殖、凋亡、迁移和 AKT 信号通路相关的分子表达水平。此外，我们使用激动剂进行了挽救实验，以验证受影响的信号通路。