通过伽马射线辐照获得的具有改良加工性状的马铃薯（L.）突变体的诱导与筛选

induction and selection of mutants obtained through gamma irradiation with improved processing traits in potato ( L.).

作者信息

Kaur Rupinder, Sharma Sat Pal, Kalia Anu, Kaur Navraj, Manchanda Pooja

机构信息

Department of Vegetable Science, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, India.

Electron Microscopy and Nanoscience Lab, Department of Soil Science, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab, India.

出版信息

Int J Radiat Biol. 2024;100(1):139-149. doi: 10.1080/09553002.2023.2245472. Epub 2023 Aug 14.

DOI:10.1080/09553002.2023.2245472

PMID:37548596

Abstract

PURPOSE

This manuscript aimed for the generation of γ-irradiation derived mutants of potato genotype PAU/RR-1501 possessing desirable processing traits.

MATERIALS AND METHODS

Nodal cuttings from virus-free explants were established on basal MS medium and irradiated with different doses (0, 5, 10 and 20 Gy) of γ-irradiation. The 5 and 10 Gy treated plantlets were multiplied and used for micro-tuber induction. Harvested micro-tubers were planted in pots for the selection and evaluation of mutants in M1V2 generation.

RESULTS

Four weeks post-treatment, plantlets (5 Gy) showed enhanced growth as compared to the control while 20 Gy treatment exhibited completely ceased shoot growth. The highest number and weight of mini-tubers per plant was recorded for 10 Gy followed by 5 Gy treatment as compared to control. The γ-irradiation treatments caused changes in the skin color and shape of M1V2 tubers.

CONCLUSION

Under the 5 Gy treatment 49.9% of clones produced exhibited cream and 8.53% brown skin color. Nine putative mutants were identified in genotype PAU/RR-1501 exhibiting promising processing traits.

摘要