Thao Le Dinh, Choi Hyorim, Choi Yunhee, Mageswari Anbazhagan, Lee Daseul, Hong Seung-Beom
Korean Agricultural Culture Collection, Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.
Plant Protection Research Institute, Duc Thang, Bac Tu Liem, Ha Noi, Vietnam.
Plant Pathol J. 2023 Aug;39(4):384-396. doi: 10.5423/PPJ.OA.05.2023.0078. Epub 2023 Aug 1.
Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with "The List of Plant Diseases in Korea", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.
尖孢炭疽菌复合种是炭疽菌属中最重要的类群之一，具有高度的物种多样性和广泛的寄主植物范围。自20世纪90年代以来，尖孢炭疽菌及相关物种已从韩国的不同植物和地点采集，并保藏于韩国国立农业科学研究院的韩国农业文化保藏中心（KACC）。这些真菌分离物以前主要基于形态特征进行鉴定，并且提供了分子数据的局限性。为了确认物种鉴定，本研究使用了KACC中的64个尖孢炭疽菌复合种分离物对6个基因座进行DNA序列分析：核糖体DNA内转录间隔区（ITS）、β-微管蛋白2（TUB2）、组蛋白-3（HIS3）、甘油醛-3-磷酸脱氢酶（GAPDH）、几丁质合成酶1（CHS-1）和肌动蛋白（ACT）。分子分析表明，它们被鉴定为6个不同的物种：佛罗里达炭疽菌（24个分离物）、睡莲炭疽菌（21个分离物）、斯氏炭疽菌（12个分离物）、菊炭疽菌（3个分离物）、羽扇豆炭疽菌（2个分离物）、戈氏炭疽菌（1个分离物）以及一个新物种候选。我们将KACC分离物的寄主与《韩国植物病害名录》、韩国以前的报告以及全球报告进行了比较，发现经过致病性测试后，韩国可能会新报告23种寄主与病原菌的组合，其中12种在世界上尚未有记录。
