Favara Gabriel Madoglio, de Oliveira Felipe Franco, Ferro Camila Geovana, Kraide Heron Delgado, Carmo Eike Yudi Nishimura, Bello Vinicius Henrique, Ribeiro-Junior Marcos Roberto, Krause-Sakate Renate, Kitajima Elliot Watanabe, Rezende Jorge Alberto Marques
Laboratory of Plant Virology, Department of Plant Pathology and Nematology, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo, Piracicaba, Brazil.
Laboratory of Plant Virology and Virus-Vector-Host Interactions, Department of Plant Protection, Faculdade de Ciências Agronômicas, São Paulo State University, Botucatu, Brazil.
Front Plant Sci. 2023 Jul 26;14:1202139. doi: 10.3389/fpls.2023.1202139. eCollection 2023.
, known as bridal bouquet, exhibiting characteristic symptoms of orthotospovirus infection were found in different localities in Brazil. Symptoms were restricted to leaves of the middle and lower thirds of a few branches of each plant. Electron microscopy, molecular analyses, and complete genome sequencing identified the orthotospovirus as groundnut ringspot virus (GRSV),member of the species . The virus was poorly transmitted mechanically to . Reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analyses performed using total RNA extracted from leaf blades, primary veins, petioles, and regions of petiole insertion on branches indicated the presence of GRSV, predominantly in the symptomatic leaf blades. Symptomatic branches propagate vegetatively, often resulting in plants expressing GRSV symptoms. In contrast, vegetative propagation of the asymptomatic branches of infected plants predominantly generates plants without GRSV symptoms. The resistance of plants to GRSV infection, restricted systemic viral movement, and expression of symptoms in infected plants suggest that this orthotospovirus does not threaten this ornamental plant.
在巴西不同地区发现了被称为“新娘花束”的植物，其表现出正番茄斑萎病毒感染的特征症状。症状仅限于每株植物少数枝条中下三分之一部分的叶片。电子显微镜检查、分子分析和全基因组测序确定该正番茄斑萎病毒为花生环斑病毒（GRSV），属于该病毒种的成员。该病毒通过机械接种很难传播到……。使用从叶片、主脉、叶柄以及叶柄在枝条上的插入部位提取的总RNA进行的逆转录聚合酶链反应（RT-PCR）和逆转录定量聚合酶链反应（RT-qPCR）分析表明，GRSV主要存在于有症状的叶片中。有症状的枝条通过营养繁殖，常常导致植株表现出GRSV症状。相比之下，感染植株无症状枝条的营养繁殖主要产生无GRSV症状的植株。植物对GRSV感染的抗性、病毒在体内有限的系统移动以及感染植株中症状的表现表明，这种正番茄斑萎病毒不会威胁这种观赏植物。
