Parveen Nuzhat, Akbarsha Mohammad Abdulkader, Latif Wani A B, Ansari Mohd Owais, Ahmad Md Fahim, Shadab G G H A
Cytogenetics and Molecular Toxicology Lab., Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
Department of Biotechnology & Microbiology, National College (Autonomous), Tiruchirappalli 620001, India.
Mutat Res Genet Toxicol Environ Mutagen. 2023 Aug-Sep;890:503661. doi: 10.1016/j.mrgentox.2023.503661. Epub 2023 Jul 24.
Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in a variety of consumer and other commercial products. Hence, man faces the risk of exposure to ZnO-NPs and the consequent adverse health effects. Mitigation/prevention of such effects using natural products has drawn the attention of scientists. Therefore, the aim of the present study has been to find the toxic effects associated with exposure to ZnO-NPs, and the protective role of the phytochemicals thymoquinone (TQ) and quercetin (QCT) in the rat model. ZnO-NPs were administered to male Wistar rats through oral route; TQ / QCT was concurrently administered through intra-peritoneal route. The response in the animal was analyzed adopting chromosomal aberration test, micronucleus test, and comet assay of bone marrow cells to assess the genotoxicity, and biochemical assays of superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), total extractable protein of liver, and reduced glutathione (GSH) of liver homogenate to monitor the changes in the antioxidant defense mechanism in response to the oxidative stress. Treatment of 300 mg/kg body weight (bw) of ZnO-NPs produced adverse effects on all aspects analyzed viz., structural chromosomal aberrations, micronuclei formation, DNA damage, SOD, catalase, lipid peroxidation, GSH, and extractable total protein of liver. Co-treatment of TQ / QCT offered protection against the toxicity induced by ZnO-NPs. The most optimum doses of TQ and QCT that offered the best protection were 18 mg/kg bw and 500 mg/kg bw, respectively. The study reveals that TQ / QCT supplementation is beneficial in the context of toxic effects of ZnO-NPs.
氧化锌纳米颗粒（ZnO-NPs）越来越多地用于各种消费品和其他商业产品中。因此，人类面临接触ZnO-NPs的风险以及随之而来的健康不良影响。利用天然产物减轻/预防此类影响已引起科学家的关注。因此，本研究的目的是找出与接触ZnO-NPs相关的毒性作用，以及植物化学物质百里醌（TQ）和槲皮素（QCT）在大鼠模型中的保护作用。通过口服途径将ZnO-NPs给予雄性Wistar大鼠；同时通过腹腔途径给予TQ/QCT。采用骨髓细胞的染色体畸变试验、微核试验和彗星试验分析动物的反应，以评估遗传毒性，并通过超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、脂质过氧化（LPO）、肝脏总可提取蛋白以及肝脏匀浆中还原型谷胱甘肽（GSH）的生化测定来监测抗氧化防御机制响应氧化应激的变化。以300mg/kg体重（bw）的剂量处理ZnO-NPs对所分析的各个方面均产生了不良影响，即结构染色体畸变、微核形成、DNA损伤、SOD、CAT、脂质过氧化、GSH以及肝脏可提取总蛋白。TQ/QCT的联合处理对ZnO-NPs诱导的毒性起到了保护作用。提供最佳保护的TQ和QCT的最适宜剂量分别为18mg/kg bw和500mg/kg bw。该研究表明，补充TQ/QCT在应对ZnO-NPs的毒性作用方面是有益的。
