Fang Yuan, Ding Di, Gu Yujia, Jia Qiwei, Zheng Qiaolin, Qian Qian, Wang Yuexing, Rao Yuchun, Mao Yijian
College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
Ball Horticultural Company, West Chicago, IL 60606, USA.
Plants (Basel). 2023 Aug 2;12(15):2853. doi: 10.3390/plants12152853.
Bacterial panicle blight, bacterial leaf streak, and bacterial brown stripe are common bacterial diseases in rice that represent global threats to stable rice yields. In this study, we used the rice variety HZ, Nekken and their 120 RIL population as experimental materials. Phenotypes of the parents and RILs were quantitatively analyzed after inoculation with , pv. , and subsp. . Genetic SNP maps were also constructed and used for QTL mapping of the quantitative traits. We located 40 QTL loci on 12 chromosomes. The analysis of disease resistance-related candidate genes in the QTL regions with high LOD value on chromosomes 1, 3, 4, and 12 revealed differential expression before and after treatment, suggesting that the identified genes mediated the variable disease resistance profiles of Huazhan and Nekken2. These results provide an important foundation for cloning bacterial-resistant QTLs of panicle blight, leaf streak, and brown stripe in rice.
细菌性穗枯病、细菌性条斑病和细菌性褐条病是水稻常见的细菌性病害，对全球水稻稳定产量构成威胁。本研究以水稻品种华占（HZ）、日本晴（Nekken）及其120个重组自交系群体为试验材料。接种稻黄单胞菌稻生致病变种（Xanthomonas oryzae pv. oryzicola）、水稻条斑病菌（Xanthomonas oryzae pv. oryzicola）和稻褐条病菌（Xanthomonas oryzae subsp. oryzicola）后，对亲本和重组自交系的表型进行了定量分析。还构建了遗传SNP图谱，并用于数量性状的QTL定位。我们在12条染色体上定位了40个QTL位点。对1号、3号、4号和12号染色体上具有高LOD值的QTL区域中与抗病性相关的候选基因进行分析，结果显示处理前后存在差异表达，这表明所鉴定的基因介导了华占和日本晴2号对不同病害的抗性谱。这些结果为克隆水稻穗枯病、条斑病和褐条病的抗细菌QTL提供了重要基础。
