Department of Orthopedic Surgery, Second Affiliated Hospital, Harbin Medical University, Harbin, China.
Department of Stomatology, Xiang An Hospital of Xiamen University, China.
Gene. 2023 Nov 30;886:147713. doi: 10.1016/j.gene.2023.147713. Epub 2023 Aug 12.
Spinal cord injury (SCI) is a traumatic condition that causes myelin destruction and neuronal death, making it challenging to reverse. In spinal cord tissue, oligodendrocyte progenitor cells and oligodendrocytes are essential for maintaining myelin morphology and axon regeneration. The decrease in oligodendrocyte lineage cells after SCI is a major factor contributing to the difficulty in restoring spinal cord function. However, there is still a lack of research on the status and intercellular communication between oligodendrocyte lineage cells after injury. The development of single-cell sequencing technology has enabled researchers to obtain highly accurate cellular transcriptional information, facilitating detailed studies of cellular subpopulations. This study delved into the cellular heterogeneity of oligodendrocyte lineage cells using a single-cell transcriptomic approach to uncover functional changes and cellular interactions during different time points after SCI. Our findings highlighted the critical roles of Psap (Prosaposin)/Gpr37l1 and Psap/Gpr37 ligand-receptor pairs among oligodendrocyte lineage cells. Furthermore, we predicted the transcription factors that may play a key regulatory role. We demonstrated for the first time that Junb acts almost exclusively in mature oligodendrocytes, which provides a potential target for the study of oligodendrocyte transcriptional mechanisms.
脊髓损伤（SCI）是一种创伤性疾病，会导致髓鞘破坏和神经元死亡，因此难以逆转。在脊髓组织中，少突胶质前体细胞和少突胶质细胞对于维持髓鞘形态和轴突再生至关重要。SCI 后少突胶质细胞谱系细胞的减少是导致脊髓功能恢复困难的主要因素之一。然而，关于损伤后少突胶质细胞谱系细胞的状态和细胞间通讯，仍缺乏研究。单细胞测序技术的发展使研究人员能够获得高度准确的细胞转录信息，从而能够详细研究细胞亚群。本研究通过单细胞转录组学方法深入研究了少突胶质细胞谱系细胞的细胞异质性，揭示了 SCI 后不同时间点的功能变化和细胞相互作用。我们的研究结果强调了 Psap（前蛋白原/ Gpr37l1）/Gpr37 配体-受体对在少突胶质细胞谱系细胞中的关键作用。此外，我们预测了可能发挥关键调节作用的转录因子。我们首次证明 Junb 在成熟的少突胶质细胞中几乎特异性表达，这为研究少突胶质细胞转录机制提供了一个潜在的靶点。
