Sautter C
Eur J Cell Biol. 1986 Aug;41(2):263-9.
The heterologous in vivo translation system of Xenopus laevis oocytes was used to translate messenger RNA isolated from water-melon cotyledons. Immunocytochemistry was used to localize the translation products in situ within the oocyte. In addition, the translation products were immunoprecipitated from homogenized oocytes, separated on SDS-polyacrylamide electrophoresis and visualized by fluorography. A variety of watermelon proteins encoded in the injected mRNA were translated within the oocytes. Among them was the mitochondrial isoenzyme of malate dehydrogenase (mtMDH). The mtMDH was correctly imported into the mitochondria of the oocytes, as detected by immunocytochemistry.
利用非洲爪蟾卵母细胞的异源体内翻译系统来翻译从西瓜子叶中分离出的信使核糖核酸。采用免疫细胞化学方法在卵母细胞内原位定位翻译产物。此外，从匀浆的卵母细胞中免疫沉淀翻译产物，在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳上进行分离，并用荧光自显影法进行观察。注入的信使核糖核酸中编码的多种西瓜蛋白在卵母细胞内被翻译。其中包括苹果酸脱氢酶的线粒体同工酶（mtMDH）。通过免疫细胞化学检测发现，mtMDH被正确导入到卵母细胞的线粒体中。
